自组装 CpG 寡脱氧核苷酸连接中空金纳米球以增强与癌症相关的免疫刺激。
Self-assembled CpG oligodeoxynucleotides conjugated hollow gold nanospheres to enhance cancer-associated immunostimulation.
机构信息
Key Laboratory of Coal Conversion & New Carbon Materials of Hubei Province, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, PR China.
Key Laboratory of Biomedical Polymers of Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
出版信息
Colloids Surf B Biointerfaces. 2019 Mar 1;175:248-255. doi: 10.1016/j.colsurfb.2018.12.001. Epub 2018 Dec 3.
To realize efficiently cellular uptake and enhance the immunostimulation, thiolated cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) were self-assembled on hollow gold nanospheres (HGNs) to form CpG-HGNs. The cellular uptake of CpG-HGNs in immune cells was studied in RAW 264.7 cells. Due to the enhanced delivery efficiency, CpG-HGNs exhibited a higher immune stimulatory activity compared with CpG ODNs, resulting in a dramatically enhanced secretion of proinflammatory cytokines. In addition, CpG-HGNs showed low cytotoxicity in RAW 264.7 cells. CpG-HGNs could potentially realize synergistic photothermal therapy and immunotherapy in vivo.
为了实现高效的细胞摄取并增强免疫刺激作用，巯基化胞嘧啶-磷酸-鸟嘌呤（CpG）寡脱氧核苷酸（ODN）自组装在中空金纳米球（HGN）上形成 CpG-HGNs。在 RAW 264.7 细胞中研究了 CpG-HGNs 在免疫细胞中的摄取情况。由于递药效率的提高，与 CpG ODNs 相比，CpG-HGNs 表现出更高的免疫刺激活性，导致促炎细胞因子的分泌显著增强。此外，CpG-HGNs 在 RAW 264.7 细胞中显示出低细胞毒性。CpG-HGNs 有可能在体内实现协同光热治疗和免疫治疗。
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