State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China; Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, Hubei 430070, China.
BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China.
Cell Host Microbe. 2018 Dec 12;24(6):817-832.e8. doi: 10.1016/j.chom.2018.11.006.
Alternatives to antibiotics for preventing diarrhea in early-weaned farm animals are sorely needed. CM piglets (a native Chinese breed) are more resistant to early-weaning stress-induced diarrhea than the commercial crossbred LY piglets. Transferring fecal microbiota, but not saline, from healthy CM into LY piglets by oral administration prior to early weaning conferred diarrhea resistance. By comparing the relative abundance of intestinal microbiota in saline and microbiota transferred LY piglets, we identified and validated Lactobacillus gasseri LA39 and Lactobacillus frumenti as two bacterial species that mediate diarrhea resistance. Diarrhea resistance depended on the bacterial secretory circular peptide gassericin A, a bacteriocin. The binding of gassericin A to Keratin 19 (KRT19) on the plasma membrane of intestinal epithelial cells was essential for enhancement of fluid absorption and decreased secretion. These findings suggest the use of L. gasseri LA39 and L. frumenti as antibiotic alternatives for preventing diarrhea in mammals.
早期断奶农场动物预防腹泻的抗生素替代品迫切需要。CM 仔猪（一种中国本土品种）比商业杂交 LY 仔猪更能抵抗早期断奶应激引起的腹泻。在早期断奶前通过口服将来自健康 CM 的粪便微生物群，而不是盐水，转移到 LY 仔猪中，可赋予其腹泻抗性。通过比较盐水中和转移的微生物群的 LY 仔猪的肠道微生物群的相对丰度，我们鉴定并验证了乳杆菌 LA39 和戊糖乳杆菌是两种介导腹泻抗性的细菌种类。腹泻抗性取决于细菌分泌的环形肽 gassericin A，一种细菌素。gassericin A 与肠上皮细胞质膜上的角蛋白 19（KRT19）的结合对于增强液体吸收和减少分泌是必需的。这些发现表明，使用乳杆菌 LA39 和戊糖乳杆菌作为抗生素替代品，可预防哺乳动物腹泻。
