Zha Andong, Tan Bie, Wang Jing, Qi Ming, Deng Yuankun, Liao Peng, Yin Yulong
Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.
Institute of Subtropical Agriculture, University of Chinese Academy of Sciences, Beijing, China.
J Sci Food Agric. 2023 Aug 30;103(11):5569-5577. doi: 10.1002/jsfa.12642. Epub 2023 May 12.
Overuse of antibiotics contributes to bacterial resistance in animals. Therefore, it is necessary to find a new way to ensure animal health and promote animal growth. This experiment was conducted to investigate the effect of mannan oligosaccharide (MOS)/vitamin E (VE)/attapulgite (APT) nanocomposites (SLK1, SLK3, SLK5) on growth performance and intestinal health in weaned piglets. Each 1 kg of SLK1, SLK3 or SLK5 contains 50 g of vitamin E, and each had a different MOS concentration [SLK1 (50 g kg MOS), SLK3 (100 g kg MOS), SLK5 (150 g kg MOS)]. In total, 135 piglets were randomly divided into five groups (normal control group, traditional antibiotic substitutes group, SLK1 group, SLK3 group and SLK5 group), and growth performance, diarrhea index, intestinal epithelial barrier function and intestinal microbial composition were measured.
SLK1 and SLK5 significantly decreased diarrhea frequency in weaned piglets (p < 0.05). Furthermore, SLK5 significantly increased survival rate of weaned piglets compared to the traditional antibiotic substitutes group (p < 0.05). SLK5 also increased villus height of ileum, and increased goblet number of the jejunum (p < 0.05). 16S rRNA sequencing showed that SLK5 significantly regulated intestinal colonic microbiota composition (p < 0.05). Specifically, SLK5 significantly increased the abundance of Phascolarctobacterium succinatutens in the cecum and increased the abundance of Lactobacillus and Bifidobacterium in the colon (p < 0.05). In addition, dietary supplementation with 1 kg T SLK5 also significantly increased the propionate content in the colon, which is significantly correlated with Phascolarctobacterium (p < 0.05).
Dietary supplementation with 1 kg T SLK5 improved intestinal epithelial barrier function, and regulated intestinal microbiota composition to prevent diarrhea in weaned piglets. © 2023 Society of Chemical Industry.
抗生素的过度使用会导致动物产生细菌耐药性。因此，有必要寻找一种新方法来确保动物健康并促进动物生长。本实验旨在研究甘露寡糖（MOS）/维生素E（VE）/凹凸棒石（APT）纳米复合材料（SLK1、SLK3、SLK5）对断奶仔猪生长性能和肠道健康的影响。每1千克SLK1、SLK3或SLK5含有50克维生素E，且每种材料的MOS浓度不同[SLK1（50克/千克MOS）、SLK3（100克/千克MOS）、SLK5（150克/千克MOS）]。总共135头仔猪被随机分为五组（正常对照组、传统抗生素替代组、SLK1组、SLK3组和SLK5组），并测定生长性能、腹泻指数、肠道上皮屏障功能和肠道微生物组成。
SLK1和SLK5显著降低了断奶仔猪的腹泻频率（p<0.05）。此外，与传统抗生素替代组相比，SLK5显著提高了断奶仔猪的存活率（p<0.05）。SLK5还增加了回肠绒毛高度，并增加了空肠杯状细胞数量（p<0.05）。16S rRNA测序表明，SLK5显著调节了肠道结肠微生物群组成（p<0.05）。具体而言，SLK5显著增加了盲肠中琥珀酸产碱菌的丰度，并增加了结肠中乳酸杆菌和双歧杆菌的丰度（p<0.05）。此外，日粮中添加1千克T SLK5还显著增加了结肠中丙酸的含量，这与产碱菌显著相关（p<0.05）。
日粮中添加1千克T SLK5可改善肠道上皮屏障功能，并调节肠道微生物群组成，以预防断奶仔猪腹泻。©2023化学工业协会。
