School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
School of Life Science, East China Normal University, Shanghai 200241, China.
Int J Mol Sci. 2018 Dec 7;19(12):3935. doi: 10.3390/ijms19123935.
, a medicinal food fungus, is a fruit from . In this study, we purified ergosterol peroxide (EP) from the fermentation broth of and investigated its effects on renal cell carcinoma (RCC) cells, in vitro. EP was purified from fermentation broth. The human RCC cell line 786-0 was used to analyze the anticancer mechanism of EP and inhibit its effect on cancer cell proliferation, in vitro. EP with a validated structure showed a yield rate of 20.1 mg/L and a purity of 96%. EP significantly inhibited RCC cell growth and clone formation in vitro. In addition, EP suppressed the migration and invasion, triggered the apoptosis, and modulated the cell cycle of RCC cells, in a dose-dependent manner. It also downregulated β-catenin expression. EP could be routinely produced through . It fights RCC cells in vitro through multiple mechanisms, including suppressing cell growth, colonization, migration, and invasion, arresting the cell cycle, attenuating β-catenin pathways, and triggering apoptosis.
灵芝，一种药用真菌，是多孔菌科灵芝属真菌的子实体。在这项研究中，我们从灵芝发酵液中纯化了麦角甾醇过氧化物（EP），并在体外研究了其对肾透明细胞癌细胞（RCC）的作用。EP 从灵芝发酵液中纯化得到。采用人肾透明细胞癌细胞系 786-0 分析 EP 的抗癌机制并抑制其对癌细胞增殖的影响。结构验证的 EP 的产率为 20.1mg/L，纯度为 96%。EP 显著抑制 RCC 细胞在体外的生长和克隆形成。此外，EP 以剂量依赖性方式抑制 RCC 细胞的迁移和侵袭，触发细胞凋亡，并调节细胞周期。它还下调了β-连环蛋白的表达。EP 可以通过常规方法生产。它通过多种机制在体外对抗 RCC 细胞，包括抑制细胞生长、定植、迁移和侵袭、阻断细胞周期、减弱β-连环蛋白途径和触发细胞凋亡。
