Kwiecińska-Piróg Joanna, Skowron Krzysztof, Gospodarek-Komkowska Eugenia
Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz , Poland.
Pol J Microbiol. 2018;67(4):471-478. doi: 10.21307/pjm-2018-055.
Proteus spp. is an etiological factor of urinary tract and bloodstream infections. The aim of this study was the retrospective analysis of susceptibility of Proteus spp. strains isolated from bloodstream infections (BSIs) as well as similarity evaluation of the strains isolated from different clinical samples. Proteus spp. strains were isolated in 2009-2017 from hospital patients. Identification was based on the colony's morphology and biochemical or MALDI-TOF MS analyzes. The antibiotic susceptibility test was done using the diffusion method. Biofilm formation was evaluated with microplate method using TTC. Bacteremia caused by Proteus spp. was found in 97 patients, mainly secondary to urinary tract infection. Most of the strains were susceptible to piperacillin with tazobactam (95.9%) and amikacin (86.7%). Elderly patients have a higher risk of mortality after BSIs caused by Proteus spp. A detailed analysis was made for randomly chosen 26 strains isolated from 11 patients with Proteus mirabilis bacteremia. Using PFGE, we found that 10 (90.9%) isolates, collected from different clinical specimens of the same patient, were genetically identical. spp. is an etiological factor of urinary tract and bloodstream infections. The aim of this study was the retrospective analysis of susceptibility of spp. strains isolated from bloodstream infections (BSIs) as well as similarity evaluation of the strains isolated from different clinical samples. spp. strains were isolated in 2009–2017 from hospital patients. Identification was based on the colony’s morphology and biochemical or MALDI-TOF MS analyzes. The antibiotic susceptibility test was done using the diffusion method. Biofilm formation was evaluated with microplate method using TTC. Bacteremia caused by spp. was found in 97 patients, mainly secondary to urinary tract infection. Most of the strains were susceptible to piperacillin with tazobactam (95.9%) and amikacin (86.7%). Elderly patients have a higher risk of mortality after BSIs caused by spp. A detailed analysis was made for randomly chosen 26 strains isolated from 11 patients with bacteremia. Using PFGE, we found that 10 (90.9%) isolates, collected from different clinical specimens of the same patient, were genetically identical.
变形杆菌属是泌尿道和血流感染的一个病因。本研究的目的是对从血流感染（BSIs）中分离出的变形杆菌属菌株的药敏性进行回顾性分析，以及对从不同临床样本中分离出的菌株进行相似性评估。变形杆菌属菌株于2009年至2017年从医院患者中分离得到。鉴定基于菌落形态以及生化或基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析。采用扩散法进行抗生素药敏试验。使用氯化三苯基四氮唑（TTC）通过微孔板法评估生物膜形成。97例患者中发现了由变形杆菌属引起的菌血症，主要继发于尿路感染。大多数菌株对哌拉西林他唑巴坦（95.9%）和阿米卡星（86.7%）敏感。老年患者在由变形杆菌属引起的血流感染后有更高的死亡风险。对从11例奇异变形杆菌菌血症患者中随机选取的26株菌株进行了详细分析。使用脉冲场凝胶电泳（PFGE），我们发现从同一患者的不同临床标本中收集的10株（90.9%）分离株在基因上是相同的。变形杆菌属是泌尿道和血流感染的一个病因。本研究的目的是对从血流感染（BSIs）中分离出的变形杆菌属菌株的药敏性进行回顾性分析，以及对从不同临床样本中分离出的菌株进行相似性评估。变形杆菌属菌株于2009年至2017年从医院患者中分离得到。鉴定基于菌落形态以及生化或基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析。采用扩散法进行抗生素药敏试验。使用氯化三苯基四氮唑（TTC）通过微孔板法评估生物膜形成。97例患者中发现了由变形杆菌属引起的菌血症，主要继发于尿路感染。大多数菌株对哌拉西林他唑巴坦（95.9%）和阿米卡星（86.7%）敏感。老年患者在由变形杆菌属引起的血流感染后有更高的死亡风险。对从11例奇异变形杆菌菌血症患者中随机选取的26株菌株进行了详细分析。使用脉冲场凝胶电泳（PFGE），我们发现从同一患者的不同临床标本中收集的10株（90.9%）分离株在基因上是相同的。
