从线粒体转录因子 A 向与胸腺嘧啶二聚体光修复相关的 DNA 注入电子。

Electron injection from mitochondrial transcription factor A to DNA associated with thymine dimer photo repair.

机构信息

Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo, Kyoto 606-8502, Japan.

Medical Research Support Center, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.

出版信息

Bioorg Med Chem. 2019 Jan 15;27(2):278-284. doi: 10.1016/j.bmc.2018.11.044. Epub 2018 Nov 29.

DOI:10.1016/j.bmc.2018.11.044

PMID:30552005

Abstract

Electron transfer through π-stacked arrays of double-stranded DNA contributes to the redox chemistry of bases, including guanine oxidation and thymine-thymine dimer repair by photolyase. 5-Bromouracil is an attractive photoreactive thymine analogue that can be used to investigate electron transfer in DNA, and is a useful probe for protein-DNA interaction analysis. In the present study using U we found that UV irradiation facilitated electron injection from mitochondrial transcription factor A into DNA. We also observed that this electron injection could lead to repair of a thymine-thymine dimer.

摘要

电子通过双链 DNA 的π堆积阵列转移有助于碱基的氧化还原化学，包括鸟嘌呤氧化和光解酶修复胸腺嘧啶-胸腺嘧啶二聚体。5-溴尿嘧啶是一种有吸引力的光反应胸腺嘧啶类似物，可用于研究 DNA 中的电子转移，并且是蛋白质-DNA 相互作用分析的有用探针。在本研究中，我们发现 U 在用 UV 照射时促进了线粒体转录因子 A 向 DNA 的电子注入。我们还观察到，这种电子注入可以导致胸腺嘧啶-胸腺嘧啶二聚体的修复。