Andreetta Corral Marcelo, de Paula Fabiana Martins, Meisel Dirce Mary C L, Abdala Edson, Figueiredo Costa Silvia, Camera Pierrotti Ligia, Yamashiro Juliana, do Nascimento Gonçalves Elenice M, Castilho Vera Lucia P, Chieffi Pedro Paulo, Gryschek Ronaldo Cesar B
Laboratório de Investigação Médica (LIM/06 - Laboratório de Imunopatologia da Esquistossomose), Hospital das Clínicas da Faculdade de Medicina, USP, São Paulo, Brazil.
Laboratório de Investigação Médica (LIM/06 - Laboratório de Imunopatologia da Esquistossomose), Hospital das Clínicas da Faculdade de Medicina, USP, São Paulo, Brazil; Instituto de Medicina Tropical de São Paulo, USP, São Paulo, Brazil.
Acta Trop. 2019 Feb;190:357-360. doi: 10.1016/j.actatropica.2018.12.020. Epub 2018 Dec 12.
Immunocompromised patients constitute a risk group for the development of severe clinical forms of human strongyloidiasis. The diagnosis of this infection is primarily performed by parasitological techniques, but with low sensitivity. Serological techniques appear as an alternative, especially with heterologous antigens use. The aim of this study was to perform the Western blot technique by using S. venezuelensis infective third stage larva (iL3) soluble (TS) and membrane (TM) saline antigens to reveal immunoreactive bands in immunocompromised patients with strongyloidiasis. Serum samples from 117 parasitologically well-characterized patients were divided into four groups: S. stercoralis positive and immunocompetent (S + IC); S. stercoralis positive and immunocompromised (S + IP); negative and immunocompetent (S-IC); negative and immunocompromised (S-IP). A 40-35 kDa band was recognized by 100% of patients in the S + IC group in both antigenic fractions, and by 62.5% and 50% in the S + IP group using the TS and TM fractions, respectively. A 29 kDa band was recognized by 86.3% and 72.7% (for TS and TM, respectively) of patients in the S + IC group, and only by 12.5% of patients in the S + IP group on the TM antigen. Regardless of the patients' immunological condition, the 40-35 kDa band from S. venezuelensis was detected more frequently and can be used as an important marker to the immunodiagnosis of human strongyloidiasis.
免疫功能低下的患者是发生严重临床型人类类圆线虫病的风险群体。这种感染的诊断主要通过寄生虫学技术进行，但敏感性较低。血清学技术似乎是一种替代方法，特别是在使用异源抗原时。本研究的目的是通过使用委内瑞拉类圆线虫感染性第三期幼虫（iL3）的可溶性（TS）和膜（TM）盐水抗原进行蛋白质印迹技术，以揭示类圆线虫病免疫功能低下患者中的免疫反应条带。将117例经寄生虫学充分表征的患者的血清样本分为四组：粪类圆线虫阳性且免疫功能正常（S + IC）；粪类圆线虫阳性且免疫功能低下（S + IP）；阴性且免疫功能正常（S-IC）；阴性且免疫功能低下（S-IP）。在两个抗原组分中，S + IC组100%的患者识别出一条40 - 35 kDa的条带，在S + IP组中，使用TS和TM组分时分别有62.5%和50%的患者识别出该条带。S + IC组中分别有86.3%和72.7%（分别针对TS和TM）的患者识别出一条29 kDa的条带，而在S + IP组中，仅12.5%的患者在TM抗原上识别出该条带。无论患者的免疫状况如何，委内瑞拉类圆线虫的40 - 35 kDa条带检测频率更高，可作为人类类圆线虫病免疫诊断的重要标志物。
