Sudré Adriana P, Siqueira Ricardo C, Barreto Magali G M, Peralta Regina H S, Macedo Heloisa W, Peralta José M
Departamento de Parasitologia e Microbiologia e, Universidade Federal Fluminense, Niterói, Rio de Janeiro 24030210, Brazil.
Parasitol Res. 2007 Sep;101(4):1117-23. doi: 10.1007/s00436-007-0596-6. Epub 2007 Jun 14.
Strongyloidiasis caused by the intestinal nematode Strongyloides stercoralis typically occurs in the asymptomatic form. The definitive diagnosis is usually done by detection of larvae on fecal samples. However, as the parasite load is often low in most cases, microscopy is not usually sensitive and specific, and diagnosis becomes extremely difficult. Thus, development of reliable serological methods is imperative. In the present study, a diversity of epitopes from S. stercoralis larva were characterized by analysis of reactivity with serum samples obtained from individuals with and without the infection by using Western blot technique. A total of 91 serum samples belonging to 5 groups were analyzed. Different reactivity profiles were observed, representing recognition of proteins with molecular mass varied from 6 to 129 kDa. A protein band of approximately 26 kDa presented a high frequency of reactivity with serum samples from the strongyloidiasis patients group (18/23). Reactivity with this protein band was also observed in only 7 of 64 non-infected individuals or individuals infected with other helminthes. Reactivity with 2 other bands, 1 of approximately 33 kDa and a duplet of approximately 21 kDa, were also found in high frequency (17/23 and 9/23, respectively). However, reactivity with these bands was also observed in all the other serum groups studied. The results indicate that the 26-kDa band maybe be an important tool for the development of diagnostic techniques for strongyloidiasis.
由肠道线虫粪类圆线虫引起的粪类圆线虫病通常以无症状形式出现。确诊通常通过检测粪便样本中的幼虫来进行。然而，由于大多数情况下寄生虫负荷往往较低，显微镜检查通常不敏感且不特异，诊断变得极其困难。因此，开发可靠的血清学方法势在必行。在本研究中，通过使用蛋白质印迹技术分析来自感染和未感染个体的血清样本的反应性，对粪类圆线虫幼虫的多种表位进行了表征。共分析了属于5组的91份血清样本。观察到不同的反应性谱，代表对分子量从6至129 kDa不等的蛋白质的识别。一条约26 kDa的蛋白带与粪类圆线虫病患者组的血清样本呈现高频率反应性（18/23）。在64名未感染个体或感染其他蠕虫的个体中，只有7人也观察到与该蛋白带的反应性。还发现与另外两条带的反应性频率较高，一条约33 kDa，一条约21 kDa的双重条带（分别为17/23和9/23）。然而，在所有其他研究的血清组中也观察到与这些条带的反应性。结果表明，26 kDa条带可能是开发粪类圆线虫病诊断技术的重要工具。
