Conway D J, Bailey J W, Lindo J F, Robinson R D, Bundy D A, Bianco A E
Wellcome Research Centre for Parasitic Infections, Department of Biology, Imperial College, London, United Kingdom.
J Infect Dis. 1993 Sep;168(3):784-7. doi: 10.1093/infdis/168.3.784.
Proteins from a deoxycholate-soluble extract of Strongyloides stercoralis infective larvae were separated by SDS-PAGE, blotted onto nitrocellulose paper, and reacted with sera from individuals with confirmed S. stercoralis infections (n = 100), suspected S. stercoralis infections in whom no larvae could be detected (n = 27), and other nematode infections (40 with Wuchereria bancrofti, 20 with Onchocerca volvulus, 20 with Necator americanus, and 20 with mixed Ascaris lumbricoides and Trichuris trichiura infections). Immunodominant proteins of approximately 41, 31, and 28 kDa were recognized by IgG in 91%, 88%, and 90%, respectively, of sera from those with confirmed strongyloidiasis; in 100%, 100%, and 93% of sera from those with suspected strongyloidiasis; and in 9%, 12%, and 14% of sera from those infected with other nematodes. IgG reactivity to each of these proteins was a more specific means of immunodiagnosis than the currently used indirect ELISA; the methods were equally sensitive.
用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离粪类圆线虫感染性幼虫的脱氧胆酸盐可溶性提取物中的蛋白质，将其印迹到硝酸纤维素纸上，并与确诊为粪类圆线虫感染的个体（n = 100）、疑似粪类圆线虫感染但未检测到幼虫的个体（n = 27）以及其他线虫感染个体（40例班氏吴策线虫感染、20例盘尾丝虫感染、20例美洲板口线虫感染以及20例蛔虫和鞭虫混合感染）的血清反应。在确诊粪类圆线虫病患者的血清中，分别有91%、88%和90%的IgG识别出约41、31和28 kDa的免疫显性蛋白；在疑似粪类圆线虫病患者的血清中，这一比例分别为100%、100%和93%；而在感染其他线虫的患者血清中，这一比例分别为9%、12%和14%。与目前使用的间接酶联免疫吸附测定（ELISA）相比，IgG对这些蛋白质的反应性是一种更特异的免疫诊断方法；两种方法的敏感性相同。
