Cunha Renata A, de Carvalho Edson F G, de Sousa José E N, Costa-Cruz Julia M
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, Bloco 4C, Uberlândia, Minas Gerais 38400-902, Brazil.
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, Bloco 4C, Uberlândia, Minas Gerais 38400-902, Brazil.
Parasitol Int. 2017 Oct;66(5):671-676. doi: 10.1016/j.parint.2017.07.001. Epub 2017 Jul 10.
Strongyloidosis is a neglected disease that affects millions of people around the world. The cases that particularly deserve attention are those related to hyperinfection, mainly in immunocompromised patients. In this sense, there is a need to improve the serological diagnosis of this helminth. The objective of this study was therefore to produce and characterize excretory/secretory (E/S) antigens of Strongyloides venezuelensis infective larvae (L3) for use as a heterologous antigen in the diagnosis of human strongyloidosis and other parasitic infection groups. Soluble antigenic preparations were produced as total saline extract (SE), E/S in Roswell Park Memorial Institute 1640 (RPMI) and E/S in phosphate buffered saline (PBS). The three antigenic preparations showed similar protein bands. An ELISA showed that the E/S antigens were profitable, easy to use, and more sensitive and specific than SE, eliminating cross-reactivity with other parasites in serum samples. The detection of anti-Strongyloides stercoralis in the sera of patients with strongyloidosis and those with immunosuppressive conditions using S. venezuelensis L3 larvae E/S antigens was satisfactory. RPMI and PBS E/S antigens were also superior in terms of specificity than SE.
类圆线虫病是一种被忽视的疾病，影响着全球数百万人。尤其值得关注的病例是那些与超感染相关的病例，主要发生在免疫功能低下的患者中。从这个意义上说，有必要改进这种蠕虫的血清学诊断方法。因此，本研究的目的是制备并表征委内瑞拉类圆线虫感染性幼虫（L3）的排泄/分泌（E/S）抗原，用作诊断人类类圆线虫病和其他寄生虫感染组的异源抗原。制备了可溶性抗原制剂，分别为总盐水提取物（SE）、罗斯韦尔公园纪念研究所1640培养基（RPMI）中的E/S以及磷酸盐缓冲盐水（PBS）中的E/S。这三种抗原制剂显示出相似的蛋白条带。酶联免疫吸附测定（ELISA）表明，E/S抗原具有实用性、易于使用，并且比SE更敏感、更特异，消除了与血清样本中其他寄生虫的交叉反应。使用委内瑞拉类圆线虫L3幼虫E/S抗原检测类圆线虫病患者和免疫抑制患者血清中的抗粪类圆线虫抗体，结果令人满意。RPMI和PBS E/S抗原在特异性方面也优于SE。
