Department of Food Science and Technology, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China; Department of Biology, Lakehead University, Ontario P7B 5E1, Canada; Co-innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Department of Biology, Lakehead University, Ontario P7B 5E1, Canada.
Bioresour Technol. 2019 Jan;272:26-33. doi: 10.1016/j.biortech.2018.09.069. Epub 2018 Sep 16.
To develop a cost-effective, time-saving and efficient saccharification system for converting biomass into mono-/oligo-saccharides for production of bioethanol or other biochemicals, a relatively low recalcitrant and widely available biomass Agave americana was selected as feedstock. During the investigation of efficient enzyme cocktail, pectinase, which usually is neglect for biomass saccharification, was confirmed that it dramatically improves the saccharification of agave biomass. A production-friendly fungal strain of Aspergillus niger Gyx086 was employed for low-cost enzyme cocktails production using wheat straw as substance. The enzyme cocktail which was with hyperactive pectinase activity of 6.29 ± 0.42 U/ml could efficiently saccharify un-pretreated agave biomasses. As a result, under a mild condition at 35 °C in less than 72 h, most of the polysaccharides were completely converted into reducing sugar. The low-cost, process-simplified, and efficient biotechnology should stimulate the development of agave as feedstock for green energy and bio-based products production.
为开发一种经济高效、省时高效的糖化系统，将生物质转化为单糖/寡糖，用于生产生物乙醇或其他生物化学品，选择相对低抗性且广泛可用的生物质龙舌兰作为原料。在研究高效酶制剂时，发现果胶酶通常被忽视，但它可显著提高龙舌兰生物质的糖化效率。利用小麦秸秆作为基质，采用生产友好型真菌黑曲霉 Gyx086 生产低成本酶制剂。该酶制剂具有超高活性的果胶酶活性，可达 6.29±0.42 U/ml，可有效糖化未经预处理的龙舌兰生物质。结果表明，在温和条件（35°C 下）不到 72 小时内，大部分多糖完全转化为还原糖。这种低成本、简化工艺、高效的生物技术应该会刺激龙舌兰作为绿色能源和生物基产品生产的原料的发展。
