拉恩木聚糖及其多糖二肽缀合物的理化性质与免疫刺激活性。
Physicochemical properties, immunostimulatory activity of the Lachnum polysaccharide and polysaccharide-dipeptide conjugates.
机构信息
Microbial Resources and Application Laboratory, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
Microbial Resources and Application Laboratory, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
出版信息
Carbohydr Polym. 2019 Feb 15;206:446-454. doi: 10.1016/j.carbpol.2018.09.067. Epub 2018 Sep 28.
The physicochemical properties and the immunoregulatory actions in vitro of an exopolysaccharide from Lachnum (LEP) and its conjugation with a dipeptide (LEP-RH) were investigated aiming to improve their functional characteristics. The structure characteristic of the LEP and LEP-RH were determined via FT-IR and NMR. The physicochemical properties were evaluated by scanning electron microscopy (SEM), rheometer, and differential scanning calorimeter (DSC). SEM results showed that LEP-RH had a rough surface and relatively loose distribution that different from LEP. Rheological studies of LEP and LEP-RH at the same concentration indicated that LEP and LEP-RH have similar shear-thinning behaviors and gel-like structures, while LEP-RH has a better thermal stability than LEP. Bioassay results showed that treatment with the higher dosage (200 μg/mL) of LEP and LEP-RH stimulated the proliferation, cytokine secretion (IL-2, IL-6 and TNF-α) of RAW264.7 macrophages.
本研究旨在改善拉氏拟多孔菌多糖（LEP）及其二肽缀合物（LEP-RH）的功能特性，对其理化性质和体外免疫调节作用进行了研究。采用傅里叶变换红外光谱（FT-IR）和核磁共振（NMR）对 LEP 和 LEP-RH 的结构特征进行了测定。通过扫描电子显微镜（SEM）、流变仪和差示扫描量热仪（DSC）评估了其理化性质。SEM 结果表明，LEP-RH 表面粗糙，分布相对疏松，与 LEP 不同。在相同浓度下，对 LEP 和 LEP-RH 的流变学研究表明，LEP 和 LEP-RH 具有相似的剪切稀化行为和凝胶状结构，而 LEP-RH 比 LEP 具有更好的热稳定性。生物测定结果表明，较高剂量（200μg/mL）的 LEP 和 LEP-RH 处理可刺激 RAW264.7 巨噬细胞的增殖和细胞因子分泌（IL-2、IL-6 和 TNF-α）。
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