Department of Chemical and Materials Engineering, National Central University, Zhongli District, Taoyuan City, Taiwan; Center for Biocellular Engineering, National Central University, Zhongli District, Taoyuan City, Taiwan.
Department of Chemical and Materials Engineering, National Central University, Zhongli District, Taoyuan City, Taiwan.
Carbohydr Polym. 2019 Feb 15;206:70-79. doi: 10.1016/j.carbpol.2018.10.087. Epub 2018 Oct 29.
Cancer stem cells (CSCs) are potential platforms to high-throughput screen anti-cancer drugs. However, they are difficult to isolate from cancer cells. Therefore, we proposed to fabricate 3-D scaffolds for CSC enrichment. Alginate is a biocompatible polysaccharide with poor cell adhesion, whereas polycaprolactone (PCL) is relative cell adhesive. These two materials were coelectrospun as composite scaffolds. Cells collected from alginate and composite fibers demonstrated high stemness, epithelial-mesenchymal transition, invasion, drug resistance, and angiogenesis. Interestingly, cells collected from composite fibers with low ratio of PCL were significantly improved their CSC properties compared to those from pure alginate fibers because few PCL fibers spatially separated cell populations to concentrate CSCs. These results suggested that alginate fibers effectively enriched CSCs and composite fibers created an uneven microenvironment to regulate cell morphology and distribution, by which cell-cell interaction was thus manipulated. These tunable scaffolds are potential to isolate CSCs from different tissues to facilitate the cancer research.
癌症干细胞（CSCs）是高通量筛选抗癌药物的潜在平台。然而，它们很难从癌细胞中分离出来。因此，我们提出了制造 3-D 支架来富集 CSCs。海藻酸盐是一种具有较差细胞黏附性的生物相容性多糖，而聚己内酯（PCL）则具有相对较好的细胞黏附性。这两种材料被共静电纺丝制成复合支架。从海藻酸盐和复合纤维中收集的细胞表现出高干性、上皮-间充质转化、侵袭、耐药性和血管生成。有趣的是，与从纯海藻酸盐纤维中收集的细胞相比，从 PCL 比例较低的复合纤维中收集的细胞显著改善了其 CSC 特性，因为少数 PCL 纤维在空间上分离了细胞群体，从而浓缩了 CSCs。这些结果表明，海藻酸盐纤维有效地富集了 CSCs，而复合纤维则创造了一个不均匀的微环境来调节细胞形态和分布，从而操纵细胞-细胞相互作用。这些可调谐支架有可能从不同组织中分离 CSCs，从而促进癌症研究。
