Department of Chemical and Materials Engineering, National Central University, Zhongli District, Taoyuan City 32001, Taiwan; Centre for Biomedical Cell Engineering, National Central University, Zhongli District, Taoyuan City 32001, Taiwan.
Department of Chemical and Materials Engineering, National Central University, Zhongli District, Taoyuan City 32001, Taiwan.
Carbohydr Polym. 2018 Mar 1;183:29-36. doi: 10.1016/j.carbpol.2017.11.030. Epub 2017 Nov 8.
Alginate and polycaprolactone (PCL) were coelectrospun using a dual-jet system to prepare composite nanofibers in defined ratios, and hence both chemical properties and hydrophobicity of scaffolds can be manipulated. These nanofibers were applied in gene immobilization: positively charged polyethyleneimine (PEI)/DNA polyplexes were adsorbed onto anionic alginate fibers, and the higher ratios of alginate resulted in the more immobilized nonviral vectors. Through the incorporation of PCL, biocompatibility of scaffolds was highly improved. Finally, these scaffolds were used for in situ transfection application. Compared to pure alginate fibers, composite fibers not only successfully transferred target genes to adhered cells but also enhanced cell morphology and viability, suggesting that alginate/PCL nanofibers were multifunctional with gene delivery capability and biocompatibility, and the manipulation of their composition can balance and optimize both requirements. To our knowledge, this approach might be the first one using electrostatic interactions to immobilize genes onto nanofibrous scaffolds for in situ transfection application.
海藻酸钠和聚己内酯（PCL）采用双射流系统共纺，以特定比例制备复合纳米纤维，从而可以控制支架的化学性质和疏水性。这些纳米纤维应用于基因固定化：带正电荷的聚乙烯亚胺（PEI）/DNA 聚阳离子复合物被吸附到阴离子海藻酸钠纤维上，海藻酸钠的比例越高，固定的非病毒载体越多。通过加入 PCL，支架的生物相容性得到了极大的提高。最后，这些支架被用于原位转染应用。与纯海藻酸钠纤维相比，复合纤维不仅成功地将靶基因转染到黏附细胞上，而且还增强了细胞形态和活力，这表明海藻酸钠/PCL 纳米纤维具有多功能性，具有基因传递能力和生物相容性，并且可以通过调整其组成来平衡和优化这两种需求。据我们所知，这种方法可能是第一种使用静电相互作用将基因固定在纳米纤维支架上用于原位转染应用的方法。
