Hu Wei-Wen, Lin Yu-Ting
Department of Chemical and Materials Engineering, National Central University, Taoyuan City, Taiwan.
Department of Chemical and Materials Engineering, National Central University, Taoyuan City, Taiwan.
Carbohydr Polym. 2022 Aug 1;289:119440. doi: 10.1016/j.carbpol.2022.119440. Epub 2022 Apr 4.
We developed a composite wound dressing that provides multifunctional wound care. Alginate and polycaprolactone (PCL) were coelectrospun as composite fibers. Highly absorbent alginate provided a moist environment for wounds and PCL increased cell adhesion. Silver nanoparticles embedded in PCL fibers for long-term release inhibited the growth of microorganisms. In addition, plasmid DNA encoding platelet-derived growth factor-B (PDGF-B) were complexed with polyethylenimine (PEI) to form cationic nanoparticles which were then adsorbed on anionic alginate fibers through electrostatic interaction. As wound cells adhered to composite fibers, they were in situ transfected to express PDGF-B continuously. Moreover, calcium ions in alginate fibers were released into the wound site through ion exchange to accelerate hemostasis. Wound healing experiments demonstrated that PDGF-B gene-loaded composite fibers accelerated wound closure and promoted collagen formation. We expect this comprehensive study offers an ideal multifunctional solution to facilitate wound healing.
我们开发了一种提供多功能伤口护理的复合伤口敷料。将藻酸盐和聚己内酯（PCL）共电纺制成复合纤维。高吸水性的藻酸盐为伤口提供了湿润环境，而PCL增强了细胞黏附。嵌入PCL纤维以实现长期释放的银纳米颗粒抑制了微生物的生长。此外，编码血小板衍生生长因子-B（PDGF-B）的质粒DNA与聚乙烯亚胺（PEI）复合形成阳离子纳米颗粒，然后通过静电相互作用吸附在阴离子藻酸盐纤维上。当伤口细胞黏附于复合纤维时，它们会被原位转染以持续表达PDGF-B。此外，藻酸盐纤维中的钙离子通过离子交换释放到伤口部位以加速止血。伤口愈合实验表明，负载PDGF-B基因的复合纤维加速了伤口闭合并促进了胶原蛋白的形成。我们期望这项综合性研究能提供一种理想的多功能解决方案以促进伤口愈合。
