Department of Pathology, Basic Sciences Institute, Universidade Federal de Minas Gerais-UFMG, Belo Horizonte, Brazil.
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais-UFMG, Belo Horizonte, Brazil.
J Oral Pathol Med. 2019 Feb;48(2):174-179. doi: 10.1111/jop.12812. Epub 2019 Jan 2.
Benign neoplasms exhibit most of the cellular phenomena considered hallmarks of cancer, except the capacity to metastasize. Thus, the elucidation of the mechanisms associated with the progression of benign neoplasms may complement and clarify the mechanisms involved in carcinogenesis. Benign odontogenic tumours often result in facial deformities and morbidities, and have complex pathogenesis, mainly due to the diversity of interactions between the odontogenic epithelium and the ectomesenchyme. Primary cell culture of such tumours is not only difficult to be established and maintained, but also tumour cells lose characteristic cellular morphology. Considering gene expression, growth, migration, proliferation and cellular morphology are controlled by cell-cell interactions and cell-extracellular matrix interactions, cell culture in 3D substrates has gained space as a way to overcome some of the limitations of traditional monolayer cell culture systems.
In this study, fragments obtained from mesenchymal odontogenic tumours were cultured in type I collagen scaffolds. Invasion tests were performed in these models, as well as phenotypic characterization of the cultured tumours.
The results obtained for the odontogenic myxoma and the cemento-ossifying fibroma demonstrate a good reproduction of the growth pattern of these tumours under ex vivo conditions. Microscopic evaluation showed maintenance of cell viability in the explants for more than 30 days, without the presence of necrosis.
This is the first study involving long-term 3D primary cultures of benign odontogenic tumours, which is expected to support complex approaches to cell and molecular biology, and to serve as an experimental model for testing molecular therapies.
良性肿瘤表现出大多数被认为是癌症标志的细胞现象，除了转移的能力。因此，阐明与良性肿瘤进展相关的机制可以补充和阐明致癌作用涉及的机制。良性牙源性肿瘤常导致面部畸形和病态，且具有复杂的发病机制，主要是由于牙源性上皮和中胚层之间的相互作用的多样性。此类肿瘤的原代细胞培养不仅难以建立和维持，而且肿瘤细胞失去特征性的细胞形态。考虑到基因表达、生长、迁移、增殖和细胞形态受细胞-细胞相互作用和细胞-细胞外基质相互作用的控制，在 3D 基质中进行细胞培养已经成为克服传统单层细胞培养系统局限性的一种方法。
在这项研究中，从间充质牙源性肿瘤中获得的组织块在 I 型胶原支架中进行培养。在这些模型中进行了侵袭试验，并对培养的肿瘤进行了表型特征分析。
牙源性粘液瘤和骨化性纤维瘤的结果表明，在体外条件下很好地再现了这些肿瘤的生长模式。显微镜评估显示，外植体中的细胞活力在 30 天以上保持，没有坏死。
这是首次涉及良性牙源性肿瘤的长期 3D 原代培养的研究，预计将支持细胞和分子生物学的复杂方法，并作为测试分子治疗的实验模型。
