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定位控制冬油菜(L.)双单倍体群体农艺性状的数量性状基因座。

Mapping QTL controlling agronomic traits in a doubled haploid population of winter oilseed rape ( L.).

作者信息

Fattahi Farshad, Fakheri Barat Ali, Solouki Mahmood, Möllers Christian, Rezaizad Abbas

机构信息

Department of Biotechnology and Plant Breeding, University of Zabol, Zabol 538-98615, Iran.

出版信息

J Genet. 2018 Dec;97(5):1389-1406.

Abstract

Identification of superior alleles for agronomic traits in genetic resources of oilseed rape ( L.) would be useful for improving the performance of locally adapted cultivars in Iran. The objective of the present work was to analyse the genetic variation and inheritance of important agronomic traits in a doubled haploid population derived from a cross between two German oilseed rape cultivars, Sansibar and Oase. Field experiments were performed in 2016-2017 with 200 doubled haploid lines and the parental genotypes applying an alpha-lattice design with two replicates. Phenological traits were recorded during the cultivation period and at maturity, seed yield, yield components and seed quality traits were determined. Significant genetic variation was found in most of the traits and heritabilities ranged from medium (48.5%) for days to end of flowering to high (92.6%) for oil content. A molecular marker linkage map was used to map 36 QTL for different traits on 17 linkage groups. Between three and four QTL were identified for each seed yield, seed weight, oil and protein content. Some of the plant material and positive QTL alleles identified for agronomic traits may be useful for improving those characters in locally adapted cultivars in Iran.

摘要

鉴定油菜(L.)遗传资源中农艺性状的优良等位基因,对于提高伊朗当地适应性品种的性能将是有用的。本研究的目的是分析由两个德国油菜品种Sansibar和Oase杂交产生的双单倍体群体中重要农艺性状的遗传变异和遗传情况。2016 - 2017年进行了田间试验,对200个双单倍体系和亲本基因型采用α - 格子设计,重复两次。在栽培期间和成熟时记录物候性状,测定种子产量、产量构成因素和种子品质性状。在大多数性状中发现了显著的遗传变异,遗传力范围从开花末期天数的中等水平(48.5%)到含油量的高水平(92.6%)。利用分子标记连锁图谱在17个连锁群上定位了36个不同性状的QTL。每个种子产量、种子重量、油和蛋白质含量鉴定出三到四个QTL。鉴定出的一些农艺性状的植物材料和正向QTL等位基因可能有助于改良伊朗当地适应性品种的这些性状。

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