Zheng Ming, Peng Cheng, Liu Hongfang, Tang Min, Yang Hongli, Li Xiaokang, Liu Jinglin, Sun Xingchao, Wang Xinfa, Xu Junfeng, Hua Wei, Wang Hanzhong
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesWuhan, China.
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural SciencesHangzhou, China.
Front Plant Sci. 2017 Jul 18;8:1246. doi: 10.3389/fpls.2017.01246. eCollection 2017.
Plant architecture is crucial for rapeseed yield and is determined by plant height (PH), branch initiation height (BIH), branch number (BN) and leaf and inflorescence morphology. In this study, we measured three major factors (PH, BIH, and BN) in a panel of 333 rapeseed accessions across 4 years. A genome-wide association study (GWAS) was performed via Q + K model and the panel was genotyped using the 60 k Infinium SNP array. We identified seven loci for PH, four for BIH, and five for BN. Subsequently, by determining linkage disequilibrium (LD) decay associated with 38 significant SNPs, we gained 31, 15, and 17 candidate genes for these traits, respectively. We also showed that PH is significantly correlated with BIH, while no other correlation was revealed. Notably, a GA signaling gene () and a flowering gene () located on chromosome A02 were identified as the most likely candidate genes associated with PH regulation. Furthermore, a meristem initiation gene () and a NAC domain transcriptional factor () that may be associated with BN were identified on the chromosome A07. This study reveals novel insight into the genetic control of plant architecture and may facilitate marker-based breeding for rapeseed.
植株形态对油菜籽产量至关重要，它由株高（PH）、分枝起始高度（BIH）、分枝数（BN）以及叶片和花序形态决定。在本研究中，我们在4年时间里对333份油菜种质资源测定了三个主要因素（PH、BIH和BN）。通过Q + K模型进行了全基因组关联研究（GWAS），并使用60k Infinium SNP芯片对该种质群体进行基因分型。我们鉴定出7个与株高相关的位点、4个与分枝起始高度相关的位点和5个与分枝数相关的位点。随后，通过确定与38个显著SNP相关的连锁不平衡（LD）衰减，我们分别获得了与这些性状相关的31个、15个和17个候选基因。我们还表明，株高与分枝起始高度显著相关，而未发现其他相关性。值得注意的是，位于A02染色体上的一个赤霉素信号基因（）和一个开花基因（）被鉴定为与株高调控最可能相关的候选基因。此外，在A07染色体上鉴定出一个可能与分枝数相关的分生组织起始基因（）和一个NAC结构域转录因子（）。本研究揭示了对植株形态遗传控制的新见解，并可能促进油菜基于标记的育种。
