Ali Mohammad A, Tsai Tsung-Han, Braun Paul V
Department of Materials Science and Engineering, Department of Chemistry, Beckman Institute for Advanced Science and Technology, and Materials Research Laboratory, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, United States.
ACS Omega. 2018 Nov 30;3(11):14665-14670. doi: 10.1021/acsomega.8b01519. Epub 2018 Nov 1.
Chemical warfare agents such as sarin are highly toxic, and detection of even trace levels is important. Using a hydrogel film containing a built-in two-dimensional chemical potential gradient, we demonstrate the detection of a sarin simulant under conditions potentially as low as a level 1 (6.90 × 10 mg/cm for 10 min) Acute Exposure Guideline Level sarin exposure. Specifically, the sarin simulant diisopropyl fluorophosphate (DFP) is aerosol-deposited on a hydrogel film containing a built-in ionic chemical gradient and the enzyme, diisopropyl fluorophosphatase (DFPase). DFPase degrades the DFP, releasing fluoride ions. The fluoride ions are then concentrated by the gradient to a miniature electrochemical sensor embedded in the hydrogel providing a 30-fold amplification of the fluoride ion signal, which is an indication of exposure to DFP, relative to a gradient-free system. This method is general for agents which hydrolyze into chemically detectable ionic species.
诸如沙林之类的化学战剂毒性极强，即使检测痕量水平也很重要。我们使用一种含有内置二维化学势梯度的水凝胶薄膜，证明了在潜在低至1级（10分钟内为6.90×10毫克/平方厘米）急性暴露指导水平沙林暴露的条件下对沙林模拟剂的检测。具体而言，将沙林模拟剂氟磷酸二异丙酯（DFP）以气溶胶形式沉积在含有内置离子化学梯度和酶二异丙基氟磷酸酶（DFPase）的水凝胶薄膜上。DFPase降解DFP，释放出氟离子。然后，氟离子通过梯度被浓缩到嵌入水凝胶中的微型电化学传感器，相对于无梯度系统，氟离子信号放大了30倍，这表明暴露于DFP。该方法对于水解成化学可检测离子物种的试剂具有通用性。
