School of Environmental and Life Sciences, University of Newcastle, Callaghan 2308, Australia.
Nat Prod Rep. 2019 Aug 14;36(8):1117-1136. doi: 10.1039/c8np00063h.
Covering: up to 2018 Marine and freshwater cyanobacteria produce a variety of toxic compounds that pose a threat to the health of humans, livestock and natural ecosystems world-wide. Significant research efforts have been directed towards understanding the biosynthesis of these cyanotoxins in an attempt to reduce their deleterious effects on water quality and, more recently, to harness their biotechnological potential. While a variety of complementary methods (such as bioinformatic analyses and isotope feeding studies) have been employed over the last three decades to address knowledge gaps in this field, this review focuses on the utility of heterologous expression and biochemical studies, including emerging technologies for engineering and expressing complete cyanotoxin gene clusters.
截至 2018 年，海洋和淡水蓝藻产生多种有毒化合物，对全球人类、牲畜和自然生态系统的健康构成威胁。为了减少这些蓝藻毒素对水质的有害影响，并在最近利用其生物技术潜力，人们投入了大量的研究努力来了解这些蓝藻毒素的生物合成。虽然在过去的三十年中，已经采用了各种互补的方法（如生物信息学分析和同位素喂养研究）来解决该领域的知识空白，但本综述侧重于异源表达和生化研究的应用，包括用于工程和表达完整蓝藻毒素基因簇的新兴技术。
