a Linyi People's Hospital , Linyi , China.
b Linyi Lanshan Center for Disease Control and Prevention , Linyi , China.
J Biomater Sci Polym Ed. 2019 Feb;30(3):190-201. doi: 10.1080/09205063.2018.1558933. Epub 2019 Jan 12.
Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care. In this study the GA-HA-CNC hydrogels were prepared by cross-linking and freeze-drying. The composition and mechanism of GA-HA-CNC hydrogels were confirmed by FTIR. The morphology and pore size were obtained by SEM. We accessed the physical property from rheological results and swelling ratio. NIH-3T3 cells were inoculated into the hydrogels and cultured for different days, then we analyzed the cytotoxicity of the prepared hydrogels by CCK-8 methods and live/dead pictorial diagram using staining kits. FTIR revealed the combination between GA, HA and CNC was attributed to the amide bond and hydrogen bonding. SEM results showed that the drying GA-HA-CNC hydrogels were spongy, with the pore diameter about 80-120 µm. CNC significantly enhanced the property of the hydrogels and play a vital role according to the rheology and swelling results. The cells culture results showed that NIH-3T3 cells can attached to, grow, and proliferate well on the GA-HA-CNC hydrogels. In conclusion, the natural GA-HA-CNC hydrogel has great potential for the skin wound repair.
明胶（GA）、透明质酸（HA）和纤维素纳米晶体（CNC）是有前途的皮肤伤口护理材料。在这项研究中，通过交联和冷冻干燥制备了 GA-HA-CNC 水凝胶。FTIR 证实了 GA-HA-CNC 水凝胶的组成和机制。SEM 获得了形态和孔径。我们从流变学结果和溶胀比评估了物理性能。将 NIH-3T3 细胞接种到水凝胶中并在不同天数培养，然后使用染色试剂盒通过 CCK-8 方法和活/死图像分析来分析制备的水凝胶的细胞毒性。FTIR 表明 GA、HA 和 CNC 之间的结合归因于酰胺键和氢键。SEM 结果表明，干燥的 GA-HA-CNC 水凝胶呈海绵状，孔径约为 80-120μm。根据流变学和溶胀结果，CNC 显著增强了水凝胶的性能并发挥了重要作用。细胞培养结果表明，NIH-3T3 细胞可以很好地附着、生长和增殖在 GA-HA-CNC 水凝胶上。总之，天然 GA-HA-CNC 水凝胶在皮肤伤口修复方面具有巨大的潜力。
