Levenson V I, Rukhadze E Z, Egorova T P, Belkin Z P, Fedosova V G
Zh Mikrobiol Epidemiol Immunobiol. 1988 Jul(7):50-4.
Shigella ribosomal vaccine was shown previously to possess protective properties in the keratoconjunctival test on guinea pigs and to be capable of preventing experimental infection in 90% of challenged monkeys. The presence of the O-specific component (OSC) constituting about 0.5% of the ribosomal preparation by serological activity suggested its importance for the protective effect. This was studied in experiments with two O-specific immunosorbents prepared by coupling anti-O rabbit antibodies with Staphylococcus aureus cells or with CNBr-Sepharose. Ribosomes treated with immunosorbents proved to be lacking the serologically active OSC and lost their ability to induce O-antibody response in rabbits and mice. After the removal of this component ribosomal preparations were incapable of ensuring protection from Shigella kerato-conjunctival infection. The isolated OSC was also inactive in this test. The data obtained in this investigation confirm the hypothesis stating that the protective activity of Shigella ribosomal vaccine is based on the combined action of ribosomes and O-specific factor whose nature and properties require further study.
志贺氏菌核糖体疫苗先前已被证明在豚鼠角膜结膜试验中具有保护特性，并且能够在90%受攻击的猴子中预防实验性感染。通过血清学活性构成核糖体制剂约0.5%的O-特异性成分（OSC）的存在表明其对保护作用的重要性。在将抗O兔抗体与金黄色葡萄球菌细胞或与溴化氰-琼脂糖偶联制备的两种O-特异性免疫吸附剂的实验中对此进行了研究。用免疫吸附剂处理过的核糖体被证明缺乏血清学活性的OSC，并且失去了在兔和小鼠中诱导O抗体反应的能力。去除该成分后，核糖体制剂无法确保预防志贺氏菌角膜结膜感染。分离出的OSC在该试验中也无活性。本研究中获得的数据证实了这一假设，即志贺氏菌核糖体疫苗的保护活性基于核糖体和O-特异性因子的联合作用，其性质和特性需要进一步研究。
