通过用核蛋白亚细胞疫苗进行肠胃外免疫来保护小鼠免受宋内志贺氏菌局部感染。
Protection against local Shigella sonnei infection in mice by parenteral immunization with a nucleoprotein subcellular vaccine.
作者信息
Levenson V J, Mallett C P, Hale T L
机构信息
Department of Enteric Infections, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100, USA.
出版信息
Infect Immun. 1995 Jul;63(7):2762-5. doi: 10.1128/iai.63.7.2762-2765.1995.
Abstract
Nucleoprotein subcellular (NPS) vaccine, consisting of ribosome-bound O polysaccharide, was prepared from avirulent Shigella sonnei. NPS vaccine was tested for safety and protective activity in the mouse intranasal challenge model of Shigella infection. The vaccine was nontoxic when injected in doses up to 10,000 micrograms, and a single subcutaneous injection of as little as 0.1 micrograms gave significant protection against a lethal intranasal challenge with S. sonnei. These data demonstrate the induction of local protective immunity by parenteral immunization, support the concept of the ribosome as a potent vaccine vector, and give additional evidence for the protective activity of the NPS vaccine against Shigella infection.
摘要
核蛋白亚细胞（NPS）疫苗由核糖体结合的O多糖组成，取自无毒的宋内志贺菌。在志贺菌感染的小鼠鼻内攻毒模型中测试了NPS疫苗的安全性和保护活性。该疫苗以高达10000微克的剂量注射时无毒，单次皮下注射低至0.1微克就能对宋内志贺菌的致死性鼻内攻毒提供显著保护。这些数据证明了通过非肠道免疫诱导局部保护性免疫，支持了核糖体作为一种有效的疫苗载体的概念，并为NPS疫苗对志贺菌感染的保护活性提供了更多证据。
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