神经元受体在质膜上表现出独立于细胞骨架的定向运动。
Neuronal Receptors Display Cytoskeleton-Independent Directed Motion on the Plasma Membrane.
作者信息
Taylor Ruth D, Heine Martin, Emptage Nigel J, Andreae Laura C
机构信息
Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London SE1 1UL, UK; MRC Centre for Neurodevelopmental Disorders, King's College London, New Hunt's House, Guy's Campus, London SE1 1UL, UK.
Leibniz Institute of Neurobiology, Research Group Molecular Physiology, Brenneckestrasse 6, Magdeburg 39118, Germany; Otto von Guericke University Magdeburg, Center for Behavioral Brain Sciences (CBBS), Universitätsplatz 2, Magdeburg 39106, Germany; Johannes Gutenberg University Mainz, Institute for Developmental Biology and Neurobiology, AG Funktional Neurobiology, Hanns-Dieter-Hüsch Weg 15, Mainz 55128, Germany.
出版信息
iScience. 2018 Dec 21;10:234-244. doi: 10.1016/j.isci.2018.12.001. Epub 2018 Dec 5.
Directed transport of transmembrane proteins is generally believed to occur via intracellular transport vesicles. However, using single-particle tracking in rat hippocampal neurons with a pH-sensitive quantum dot probe that specifically reports surface movement of receptors, we have identified a subpopulation of neuronal EphB2 receptors that exhibit directed motion between synapses within the plasma membrane itself. This receptor movement occurs independently of the cytoskeleton but is dependent on cholesterol and is regulated by neuronal activity.
一般认为跨膜蛋白的定向运输是通过细胞内运输囊泡进行的。然而，我们利用对pH敏感的量子点探针在大鼠海马神经元中进行单粒子追踪，该探针专门报告受体的表面运动，我们发现了神经元EphB2受体的一个亚群，它们在质膜本身的突触之间表现出定向运动。这种受体运动独立于细胞骨架，但依赖于胆固醇，并受神经元活动调节。
Zotero 插件