Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, China.
State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Collaborative Innovation Center for Cancer Medicine, Guangzhou 510060, China.
Int J Mol Sci. 2018 Dec 14;19(12):4049. doi: 10.3390/ijms19124049.
The development of a multifunctional nanoprobe capable of non-invasive multimodal imaging is crucial for precise tumour diagnosis. Herein, we report a facile polymer-assisted method to produce Au-Fe₃O₄ nanocomposites (NCPs) for the dual-modal magnetic resonance (MR) and X-ray computed tomography (CT) imaging of tumours. In this approach, amino-functionalized Au nanospheres were first obtained by surface modification of the bifunctional polymer SH-PEG-NH₂. Hydrophilic and carboxyl-functionalized Fe₃O₄ nanoparticles were produced by phase transfer of reverse micelle oxidation in our previous work. The Au nanoparticles were conjugated with hydrophilic Fe₃O₄ nanoparticles through an amide reaction. The obtained Au-Fe₃O₄ nanocomposites display a high r₂ relativity (157.92 mM s) and a Hounsfield units (HU) value (270 HU) at Au concentration of 8 mg/mL and could be applied as nanoprobes for the dual-modal MR/CT imaging of a xenografted tumour model. Our work provides a facile method to prepare Au-Fe₃O₄ nanocomposites for dual-modal MR/CT imaging, and this method can be extended to prepare other multifunctional nanoparticles for multimodal bioimaging.
开发一种能够进行非侵入式多模态成像的多功能纳米探针对于精确的肿瘤诊断至关重要。在此，我们报告了一种简便的聚合物辅助方法，用于制备 Au-Fe₃O₄纳米复合材料（NCPs），用于肿瘤的双模态磁共振（MR）和 X 射线计算机断层扫描（CT）成像。在该方法中，首先通过双官能聚合物 SH-PEG-NH₂的表面修饰获得氨基功能化的 Au 纳米球。在我们之前的工作中，通过反胶束氧化的相转移制备了亲水性和羧基功能化的 Fe₃O₄纳米颗粒。通过酰胺反应将 Au 纳米颗粒与亲水性 Fe₃O₄纳米颗粒偶联。所得的 Au-Fe₃O₄纳米复合材料在 Au 浓度为 8mg/mL 时表现出高 r₂相关性（157.92mM s）和 Hounsfield 单位（HU）值（270 HU），可作为双模态 MR/CT 成像的纳米探针应用于异种移植肿瘤模型。我们的工作提供了一种简便的方法来制备用于双模态 MR/CT 成像的 Au-Fe₃O₄纳米复合材料，并且该方法可以扩展到制备用于多模态生物成像的其他多功能纳米颗粒。
