• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在三阴性乳腺癌中过表达，其体外消除导致促癌表型的抑制。

Is Overexpressed in Triple Negative Breast Cancer and Its In Vitro Extinction Leads to the Inhibition of Pro-Cancer Phenotypes.

作者信息

Claude-Taupin Aurore, Fonderflick Leïla, Gauthier Thierry, Mansi Laura, Pallandre Jean-René, Borg Christophe, Perez Valérie, Monnien Franck, Algros Marie-Paule, Vigneron Marc, Adami Pascale, Delage-Mourroux Régis, Peixoto Paul, Herfs Michael, Boyer-Guittaut Michaël, Hervouet Eric

机构信息

INSERM, EFS BFC, UMR1098, Interactions Hôte-Greffon-Tumeur/Ingénierie Cellulaire et Génique, University Bourgogne Franche-Comté F-25000 Besançon, France.

Department of Pathology, University Hospital of Besançon, F-25000 Besançon, France.

出版信息

Cells. 2018 Dec 6;7(12):248. doi: 10.3390/cells7120248.

DOI:10.3390/cells7120248
PMID:30563263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6316331/
Abstract

Early detection and targeted treatments have led to a significant decrease in mortality linked to breast cancer (BC), however, important issues need to be addressed in the future. One of them will be to find new triple negative breast cancer (TNBC) therapeutic strategies, since none are currently efficiently targeting this subtype of BC. Since numerous studies have reported the possibility of targeting the autophagy pathway to treat or limit cancer progression, we analyzed the expression of six autophagy genes (, , , , and ) in breast cancer tissue, and compared their expression with healthy adjacent tissue. In our study, we observed an increase in mRNA expression in TNBC samples from our breast cancer cohort. We also showed that this increase of the transcript was confirmed at the protein level on paraffin-embedded tissues. To corroborate these in vivo data, we designed shRNA- and CRISPR/Cas9-driven inhibition of expression in the triple negative breast cancer cell line MDA-MB-436, in order to determine its role in the regulation of cancer phenotypes. We found that inhibition led to an inhibition of in vitro cancer features, suggesting that ATG9A can be considered as a new marker of TNBC and might be considered in the future as a target to develop new specific TNBC therapies.

摘要

早期检测和靶向治疗已使乳腺癌（BC）相关死亡率显著下降，然而，未来仍有重要问题需要解决。其中之一将是寻找新的三阴性乳腺癌（TNBC）治疗策略，因为目前尚无有效的方法针对这种BC亚型。由于众多研究报道了靶向自噬途径治疗或限制癌症进展的可能性，我们分析了六种自噬基因（ 、 、 、 、 和 ）在乳腺癌组织中的表达，并将其与相邻健康组织的表达进行比较。在我们的研究中，我们观察到乳腺癌队列中TNBC样本中 mRNA表达增加。我们还表明，在石蜡包埋组织的蛋白质水平上证实了该转录本的增加。为了证实这些体内数据，我们设计了shRNA和CRISPR/Cas9驱动的对三阴性乳腺癌细胞系MDA-MB-436中 表达的抑制，以确定其在癌症表型调控中的作用。我们发现 抑制导致体外癌症特征的抑制，这表明ATG9A可被视为TNBC的新标志物，未来可能被视为开发新的特异性TNBC疗法的靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1631/6316331/4def09d6c17e/cells-07-00248-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1631/6316331/82f7f84c21e2/cells-07-00248-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1631/6316331/a58555b05091/cells-07-00248-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1631/6316331/7a19d51f7a07/cells-07-00248-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1631/6316331/4def09d6c17e/cells-07-00248-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1631/6316331/82f7f84c21e2/cells-07-00248-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1631/6316331/a58555b05091/cells-07-00248-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1631/6316331/7a19d51f7a07/cells-07-00248-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1631/6316331/4def09d6c17e/cells-07-00248-g004.jpg

相似文献

1
Is Overexpressed in Triple Negative Breast Cancer and Its In Vitro Extinction Leads to the Inhibition of Pro-Cancer Phenotypes.在三阴性乳腺癌中过表达，其体外消除导致促癌表型的抑制。
Cells. 2018 Dec 6;7(12):248. doi: 10.3390/cells7120248.
2
Aberrant methylation of ATG2B, ATG4D, ATG9A and ATG9B CpG island promoter is associated with decreased mRNA expression in sporadic breast carcinoma.ATG2B、ATG4D、ATG9A和ATG9B基因启动子CpG岛的异常甲基化与散发性乳腺癌中mRNA表达降低有关。
Gene. 2016 Sep 30;590(2):285-92. doi: 10.1016/j.gene.2016.05.036. Epub 2016 Jun 3.
3
Combination of the novel histone deacetylase inhibitor YCW1 and radiation induces autophagic cell death through the downregulation of BNIP3 in triple-negative breast cancer cells in vitro and in an orthotopic mouse model.新型组蛋白去乙酰化酶抑制剂YCW1与放疗联合通过下调三阴性乳腺癌细胞中BNIP3在体外和原位小鼠模型中诱导自噬性细胞死亡。
Mol Cancer. 2016 Jun 10;15(1):46. doi: 10.1186/s12943-016-0531-5.
4
The sigma-2 receptor as a therapeutic target for drug delivery in triple negative breast cancer.σ-2受体作为三阴性乳腺癌药物递送的治疗靶点。
Biochem Biophys Res Commun. 2015 Nov 27;467(4):1070-5. doi: 10.1016/j.bbrc.2015.09.157. Epub 2015 Oct 9.
5
Lipid nanocarriers of a lipid-conjugated estrogenic derivative inhibit tumor growth and enhance cisplatin activity against triple-negative breast cancer: pharmacokinetic and efficacy evaluation.脂质共轭雌激素衍生物的脂质纳米载体抑制肿瘤生长并增强顺铂对三阴性乳腺癌的活性：药代动力学和疗效评估。
Mol Pharm. 2015 Apr 6;12(4):1105-20. doi: 10.1021/mp5008629. Epub 2015 Mar 2.
6
Inhibition of autophagy as a new means of improving chemotherapy efficiency in high-LC3B triple-negative breast cancers.抑制自噬作为提高高LC3B三阴性乳腺癌化疗效率的新手段。
Autophagy. 2014;10(12):2122-42. doi: 10.4161/15548627.2014.981788.
7
MicroRNA-101 inhibits cell progression and increases paclitaxel sensitivity by suppressing MCL-1 expression in human triple-negative breast cancer.微小RNA-101通过抑制人三阴性乳腺癌中MCL-1的表达来抑制细胞进展并增加紫杉醇敏感性。
Oncotarget. 2015 Aug 21;6(24):20070-83. doi: 10.18632/oncotarget.4039.
8
MicroRNA-455-3p promotes invasion and migration in triple negative breast cancer by targeting tumor suppressor EI24.微小RNA-455-3p通过靶向肿瘤抑制因子EI24促进三阴性乳腺癌的侵袭和迁移。
Oncotarget. 2017 Mar 21;8(12):19455-19466. doi: 10.18632/oncotarget.14307.
9
Preclinical evaluation of cyclin dependent kinase 11 and casein kinase 2 survival kinases as RNA interference targets for triple negative breast cancer therapy.细胞周期蛋白依赖性激酶11和酪蛋白激酶2存活激酶作为三阴性乳腺癌治疗的RNA干扰靶点的临床前评估
Breast Cancer Res. 2015;17:19. doi: 10.1186/s13058-015-0524-0. Epub 2015 Feb 11.
10
Thymoquinone inhibits cell proliferation, migration, and invasion by regulating the elongation factor 2 kinase (eEF-2K) signaling axis in triple-negative breast cancer.姜黄素通过调节伸长因子 2 激酶（eEF-2K）信号轴抑制三阴性乳腺癌细胞增殖、迁移和侵袭。
Breast Cancer Res Treat. 2018 Oct;171(3):593-605. doi: 10.1007/s10549-018-4847-2. Epub 2018 Jul 3.

引用本文的文献

1
Autophagy: Shedding Light on the Mechanisms and Multifaceted Roles in Cancers.自噬：揭示癌症中的机制及多方面作用
Biomolecules. 2025 Jun 22;15(7):915. doi: 10.3390/biom15070915.
2
Progress on multifunctional transmembrane protein ATG9A.多功能跨膜蛋白ATG9A的研究进展
Cell Commun Signal. 2025 Jul 1;23(1):314. doi: 10.1186/s12964-025-02317-6.
3
p.Phe508del-CFTR Trafficking: A Protein Quality Control Perspective Through UPR, UPS, and Autophagy.p.Phe508del-囊性纤维化跨膜传导调节因子转运：从未折叠蛋白反应、泛素-蛋白酶体系统及自噬角度看蛋白质质量控制

本文引用的文献

1
Targeting autophagy in cancer.靶向治疗癌症中的自噬作用。
Cancer. 2018 Aug;124(16):3307-3318. doi: 10.1002/cncr.31335. Epub 2018 Apr 19.
2
Role of autophagy in breast cancer and breast cancer stem cells (Review).自噬在乳腺癌和乳腺癌干细胞中的作用（综述）。
Int J Oncol. 2018 Apr;52(4):1057-1070. doi: 10.3892/ijo.2018.4270. Epub 2018 Feb 8.
3
STAT3-induced lncRNA HAGLROS overexpression contributes to the malignant progression of gastric cancer cells via mTOR signal-mediated inhibition of autophagy.STAT3 诱导的长链非编码 RNA HAGLROS 过表达通过 mTOR 信号介导的自噬抑制促进胃癌细胞的恶性进展。
Int J Mol Sci. 2025 Apr 11;26(8):3623. doi: 10.3390/ijms26083623.
4
Autophagy Modulation in Therapeutic Strategy of Breast Cancer Drug Resistance.自噬调节在乳腺癌耐药治疗策略中的作用
J Cancer. 2024 Aug 19;15(16):5462-5476. doi: 10.7150/jca.97775. eCollection 2024.
5
Novel exosomal circEGFR facilitates triple negative breast cancer autophagy via promoting TFEB nuclear trafficking and modulating miR-224-5p/ATG13/ULK1 feedback loop.新型外泌体 circEGFR 通过促进 TFEB 核易位和调节 miR-224-5p/ATG13/ULK1 反馈环促进三阴性乳腺癌自噬。
Oncogene. 2024 Mar;43(11):821-836. doi: 10.1038/s41388-024-02950-4. Epub 2024 Jan 27.
6
ATG9A modulated by miR-195-5p can boost the malignant progression of cervical cancer cells.miR-195-5p 调控 ATG9A 促进宫颈癌恶性进展。
Epigenetics. 2023 Dec;18(1):2257538. doi: 10.1080/15592294.2023.2257538. Epub 2023 Oct 2.
7
Inhibition of Dipeptidyl Peptidase-4 Activates Autophagy to Promote Survival of Breast Cancer Cells via the mTOR/HIF-1α Pathway.二肽基肽酶-4的抑制通过mTOR/HIF-1α途径激活自噬以促进乳腺癌细胞存活。
Cancers (Basel). 2023 Sep 12;15(18):4529. doi: 10.3390/cancers15184529.
8
Nanomaterial-assisted CRISPR gene-engineering - A hallmark for triple-negative breast cancer therapeutics advancement.纳米材料辅助的CRISPR基因工程——三阴性乳腺癌治疗进展的一个标志。
Mater Today Bio. 2022 Oct 4;16:100450. doi: 10.1016/j.mtbio.2022.100450. eCollection 2022 Dec.
9
Inhibition of autophagy by chloroquine prevents resistance to PI3K/AKT inhibitors and potentiates their antitumor effect in combination with paclitaxel in triple negative breast cancer models.氯喹通过抑制自噬作用防止三阴性乳腺癌模型对 PI3K/AKT 抑制剂产生耐药性，并增强其与紫杉醇联合的抗肿瘤作用。
J Transl Med. 2022 Jun 27;20(1):290. doi: 10.1186/s12967-022-03462-z.
10
Construction and Validation of a Prognostic Risk Model for Triple-Negative Breast Cancer Based on Autophagy-Related Genes.基于自噬相关基因的三阴性乳腺癌预后风险模型的构建与验证
Front Oncol. 2022 Feb 4;12:829045. doi: 10.3389/fonc.2022.829045. eCollection 2022.
Mol Cancer. 2018 Jan 12;17(1):6. doi: 10.1186/s12943-017-0756-y.
4
Cancer statistics, 2018.癌症统计数据，2018 年。
CA Cancer J Clin. 2018 Jan;68(1):7-30. doi: 10.3322/caac.21442. Epub 2018 Jan 4.
5
GABARAPL1 acts as a potential marker and promotes tumor proliferation and metastasis in triple negative breast cancer.GABARAPL1作为一种潜在标志物，促进三阴性乳腺癌的肿瘤增殖和转移。
Oncotarget. 2017 Aug 10;8(43):74519-74526. doi: 10.18632/oncotarget.20159. eCollection 2017 Sep 26.
6
TMEM74 promotes tumor cell survival by inducing autophagy via interactions with ATG16L1 and ATG9A.跨膜蛋白74（TMEM74）通过与自噬相关蛋白16样蛋白1（ATG16L1）和自噬相关蛋白9A（ATG9A）相互作用诱导自噬，从而促进肿瘤细胞存活。
Cell Death Dis. 2017 Aug 31;8(8):e3031. doi: 10.1038/cddis.2017.370.
7
GABARAPL1 tumor suppressive function is independent of its conjugation to autophagosomes in MCF-7 breast cancer cells.在MCF-7乳腺癌细胞中，GABARAPL1的肿瘤抑制功能独立于其与自噬体的结合。
Oncotarget. 2017 Jul 27;8(34):55998-56020. doi: 10.18632/oncotarget.19639. eCollection 2017 Aug 22.
8
Regulation of hypoxia-induced autophagy in glioblastoma involves ATG9A.胶质母细胞瘤中缺氧诱导自噬的调节涉及自噬相关蛋白9A（ATG9A）。
Br J Cancer. 2017 Sep 5;117(6):813-825. doi: 10.1038/bjc.2017.263. Epub 2017 Aug 10.
9
Proteomic signatures reveal a dualistic and clinically relevant classification of anal canal carcinoma.蛋白质组学特征揭示了肛门管癌的双重且具有临床相关性的分类。
J Pathol. 2017 Mar;241(4):522-533. doi: 10.1002/path.4858. Epub 2017 Jan 27.
10
miRpower: a web-tool to validate survival-associated miRNAs utilizing expression data from 2178 breast cancer patients.miRpower：一种利用2178例乳腺癌患者的表达数据来验证与生存相关的微小RNA的网络工具。
Breast Cancer Res Treat. 2016 Dec;160(3):439-446. doi: 10.1007/s10549-016-4013-7. Epub 2016 Oct 15.