Kogata Naoko, Bland Philip, Tsang Mandy, Oliemuller Erik, Lowe Anne, Howard Beatrice A
1The Breast Cancer Now Toby Robins Research Centre, Division of Breast Cancer Research, The Institute of Cancer Research, London, UK.
2Present Address: Cellular Signalling and Cytoskeletal Function Lab, The Francis Crick Institute, London, UK.
Commun Biol. 2018 Dec 13;1:228. doi: 10.1038/s42003-018-0215-3. eCollection 2018.
Embryonic mammary cells are a unique population comprised of undifferentiated, highly plastic progenitor cells that create normal mammary tissues. The mammary gland continues to develop after birth from descendants of embryonic mammary cells. Here, we establish cell lines from mouse mammary organs, immediately after they formed during prenatal development, to facilitate studies of primitive mammary cells, which are difficult to isolate in sufficient quantities for use in functional experiments. We show that some lines can be induced to secrete milk, a distinguishing feature of mammary epithelial cells. Targeted deletion of , from one clone, decreases the ability to respond to lactogenic stimuli, consistent with a previously identified role for in regulating luminal progenitor function. ablation also leads to alterations in 3D morphology and downregulation of Zeb1, a key epithelial-mesenchymal transition regulator. Prenatal mammary cell lines are an invaluable resource to study regulation of mammary progenitor cell biology and development.
胚胎乳腺细胞是一个独特的细胞群体，由未分化的、具有高度可塑性的祖细胞组成，这些祖细胞可形成正常的乳腺组织。出生后，乳腺从胚胎乳腺细胞的后代继续发育。在此，我们在小鼠乳腺器官于产前发育过程中刚形成后，就从中建立细胞系，以促进对原始乳腺细胞的研究，这些原始乳腺细胞难以大量分离用于功能实验。我们发现一些细胞系可被诱导分泌乳汁，这是乳腺上皮细胞的一个显著特征。从一个克隆中靶向缺失 ，会降低对催乳刺激的反应能力，这与之前确定的 在调节管腔祖细胞功能中的作用一致。 缺失还会导致三维形态的改变以及关键上皮 - 间质转化调节因子Zeb1的下调。产前乳腺细胞系是研究乳腺祖细胞生物学和发育调控的宝贵资源。
