中国流行的鸭腺病毒3型的分离与鉴定

Shi Shaohua, Liu Rongchang, Wan Chunhe, Cheng Longfei, Chen Zhen, Fu Guanghua, Chen Hongmei, Fu Qiuling, Huang Yu

Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, 247 Wusi Road, Fuzhou, Fujian, People's Republic of China.

Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian, People's Republic of China.

Arch Virol. 2019 Mar;164(3):847-851. doi: 10.1007/s00705-018-4105-2. Epub 2018 Dec 18.

Recently, infectious disease outbreaks characterized by swelling and hemorrhagic liver and kidneys occurred in Muscovy ducklings in China. Four viruses were isolated and identified as adenoviruses by transmission electron microscopy (TEM) and polymerase chain reaction (PCR). Sequence analysis identified the new isolates as duck adenovirus 3 (DAdV-3), species Duck aviadenovirus B. The pathogenicity of the new isolate DAdV-3 FJGT01 was investigated using challenge experiments. The gross lesions in the animal experiment were similar to the clinical lesions observed in the diseased ducks. TEM examination of liver sample showed that virions accumulated and arranged in crystal lattice formations in the nuclei of hepatocytes. The present study provides new information about the epidemiology and characteristics of duck adenovirus associated with Muscovy ducklings.

最近，中国番鸭雏鸭中出现了以肝脏和肾脏肿胀及出血为特征的传染病暴发。通过透射电子显微镜（TEM）和聚合酶链反应（PCR）分离并鉴定出4种病毒为腺病毒。序列分析确定新分离株为鸭腺病毒3型（DAdV-3），属于鸭禽腺病毒B种。通过攻毒实验研究了新分离株DAdV-3 FJGT01的致病性。动物实验中的大体病变与患病鸭观察到的临床病变相似。肝脏样本的TEM检查显示，病毒粒子在肝细胞核中积累并呈晶格状排列。本研究提供了与番鸭雏鸭相关的鸭腺病毒流行病学和特征的新信息。