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用于抗菌活性的氧化铁纳米颗粒的合成、表征及应用

Synthesis, characterization and use of iron oxide nano particles for antibacterial activity.

作者信息

Saqib Saddam, Munis Muhammad Farooq Hussain, Zaman Wajid, Ullah Fazal, Shah Syed Nasar, Ayaz Asma, Farooq Muhammad, Bahadur Saraj

机构信息

Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

Mohi-ud-din Islamic University, Azad Jammu and Kashmir, Pakistan.

出版信息

Microsc Res Tech. 2019 Apr;82(4):415-420. doi: 10.1002/jemt.23182. Epub 2018 Dec 19.

Abstract

In this decade, the use of nano particles (NPs) against bacterial growth is increasing day by day due to remarkable alternative properties compared to molecular antibiotics. Thus, the use of iron oxide nanoparticles (IONPs) has proven one of the most important transition metals oxide-based remedy in nanotechnological advances and biological applications due to enriched biocompatibility of iron. In this study synthesis of IONPs was carried out via co-precipitation method. The crystallographic morphology of the synthesized particles was studied via X-ray diffraction which revealed cubic structure of the particles, whereas, the spinal shaped morphology of the prepared NPs was confirmed from scanning electron microscopy. Likewise, the presence of the major elements in the sample was determined through energy dispersive X-ray analysis characterization. Bactericidal effect of the NPs was assessed at pre-defined concentrations (50 and 100 μg/ml) against Gram +ve bacteria Staphylococcus aureus, Gram -ve bacteria Shigella dysentry and Escherichia coli. Bacterial strains, which demonstrate the potential of NPs. The purpose of this study was assessing the structure of the synthesized NPs for protective effect against harmful bacterial activity.

摘要

在这十年中,与分子抗生素相比,由于具有显著的替代特性,纳米颗粒(NPs)在抑制细菌生长方面的应用日益增加。因此,由于铁具有丰富的生物相容性,氧化铁纳米颗粒(IONPs)已被证明是纳米技术进步和生物应用中基于过渡金属氧化物的最重要疗法之一。在本研究中,通过共沉淀法合成了IONPs。通过X射线衍射研究了合成颗粒的晶体形态,结果表明颗粒为立方结构,而通过扫描电子显微镜证实了所制备的NPs为脊柱状形态。同样,通过能量色散X射线分析表征确定了样品中主要元素的存在。在预定义浓度(50和100μg/ml)下评估了NPs对革兰氏阳性菌金黄色葡萄球菌、革兰氏阴性菌痢疾志贺氏菌和大肠杆菌的杀菌效果。这些细菌菌株显示了NPs的潜力。本研究的目的是评估合成NPs的结构对有害细菌活性的保护作用。

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