Department of Industrial Chemistry "Toso Montanari", University of Bologna, UOS Campus di Rimini, Via dei Mille 39, 47921, Rimini, Italy.
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze bd.16 and 17, 90128, Palermo, Italy.
J Biol Inorg Chem. 2018 May;23(3):385-398. doi: 10.1007/s00775-018-1544-x. Epub 2018 Feb 24.
This paper deals with the synthesis and characterization of iron-hexacyanocobaltate (FeHCC) and its antibacterial properties. The nanoparticles were prepared by a facile co-precipitation technique. Crystal structure, particle morphology, and elemental composition were determined using X-ray Powder Diffraction, X-ray fluorescence spectroscopy, Transmission Electron Microscopy (TEM), and Infrared Spectroscopy (IR). The antibacterial activity of the FeHCC nanoparticles was tested against Escherichia coli and Staphylococcus aureus as models for Gram-negative and Gram-positive bacteria, respectively, by bacterial counting method and microscopic visualization (TEM, FEG-SEM, and fluorescence microscopy). The results showed that the FeHCC nanoparticles bind to the bacterial cells, inhibit bacterial growth in a dose- and time-dependent manner, inducing a loss of the membrane potential, the production of reactive oxygen species and the release of macromolecules (nucleic acids and proteins) in the extracellular environment. To the best of our knowledge, this is the first study reporting the antimicrobial effects of metal-hexacyanometallates suggesting practical uses of these materials in different areas, such as self-cleaning surfaces or food packaging.
本文研究了铁六氰合钴酸盐（FeHCC）的合成与表征及其抗菌性能。采用简便的共沉淀法制备了纳米粒子。通过 X 射线粉末衍射、X 射线荧光光谱、透射电子显微镜（TEM）和红外光谱（IR）确定了晶体结构、颗粒形态和元素组成。通过细菌计数法和微观可视化（TEM、FEG-SEM 和荧光显微镜），以大肠杆菌和金黄色葡萄球菌分别作为革兰氏阴性菌和革兰氏阳性菌的模型，测试了 FeHCC 纳米粒子的抗菌活性。结果表明，FeHCC 纳米粒子与细菌细胞结合，以剂量和时间依赖的方式抑制细菌生长，导致膜电位丧失、产生活性氧物质，并在细胞外环境中释放大分子（核酸和蛋白质）。据我们所知，这是首次报道金属六氰合金属酸盐的抗菌作用的研究，表明这些材料在自清洁表面或食品包装等不同领域具有实际用途。
