Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
J Cell Mol Med. 2019 Jan;23(1):29-38. doi: 10.1111/jcmm.13658. Epub 2018 Nov 22.
This study was designed to detecting the influences of lncRNA MEG3 in prostate cancer. Aberrant lncRNAs expression profiles of prostate cancer were screened by microarray analysis. The qRT-PCR and Western blot were employed to investigating the expression levels of lncRNA MEG3, miR-9-5p and QKI-5. The luciferase reporter assay was utilized to testifying the interactions relationship among these molecules. Applying CCK-8 assay, wound healing assay, transwell assay and flow cytometry in turn, the cell proliferation, migration and invasion abilities as well as apoptosis were measured respectively. LncRNA MEG3 was a down-regulated lncRNA in prostate cancer tissues and cells and could inhibit the expression of miR-9-5p, whereas miR-9-5p down-regulated QKI-5 expression. Overexpressed MEG3 and QKI-5 could decrease the abilities of proliferation, migration and invasion in prostate cancer cells effectively and increased the apoptosis rate. On the contrary, miR-9-5p mimics presented an opposite tendency in prostate cancer cells. Furthermore, MEG3 inhibited tumour growth and up-regulated expression of QKI-5 in vivo. LncRNA MEG3 was a down-regulated lncRNA in prostate cancer and impacted the abilities of cell proliferation, migration and invasion, and cell apoptosis rate, this regulation relied on regulating miR-9-5p and its targeting gene QKI-5.
本研究旨在检测长非编码 RNA MEG3 对前列腺癌的影响。通过微阵列分析筛选前列腺癌中异常表达的长非编码 RNA 谱。采用 qRT-PCR 和 Western blot 检测 lncRNA MEG3、miR-9-5p 和 QKI-5 的表达水平。应用荧光素酶报告基因检测这些分子之间的相互作用关系。依次采用 CCK-8 检测、划痕愈合检测、Transwell 检测和流式细胞术，分别测定细胞增殖、迁移和侵袭能力以及细胞凋亡。lncRNA MEG3 在前列腺癌组织和细胞中呈下调表达，可抑制 miR-9-5p 的表达，而 miR-9-5p 下调 QKI-5 的表达。过表达 MEG3 和 QKI-5 可有效降低前列腺癌细胞的增殖、迁移和侵袭能力，并增加细胞凋亡率。相反，miR-9-5p 模拟物在前列腺癌细胞中呈现相反的趋势。此外，MEG3 在体内抑制肿瘤生长并上调 QKI-5 的表达。lncRNA MEG3 在前列腺癌中呈下调表达，影响细胞增殖、迁移和侵袭能力以及细胞凋亡率，这种调节依赖于调节 miR-9-5p 及其靶基因 QKI-5。
