Jing Aihua, Zhang Chunxin, Liang Gaofeng, Feng Wenpo, Tian Zhengshan, Jing Chenhuan
School of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, China.
Medical College, Henan University of Science and Technology, Luoyang 471023, China.
Micromachines (Basel). 2018 Dec 18;9(12):669. doi: 10.3390/mi9120669.
Electrochemical sensors for early tumor cell detection are currently an important area of research, as this special region directly improves the efficiency of cancer treatment. Functional graphene is a promising alternative for selective recognition and capture of target cancer cells. In our work, an effective cytosensor of hyaluronate-functionalized graphene (HG) was prepared through chemical reduction of graphene oxide. The as-prepared HG nanostructures were characterized with Fourier transform infrared spectroscopy and transmission electron microscopy coupled with cyclic voltammograms and electrochemical impedance spectroscopy, respectively. The self-assembly of HG with ethylene diamine, followed by sodium hyaluronate, enabled the fabrication of a label-free electrochemical impedance spectroscopy cytosensor with high stability and biocompatibility. Finally, the proposed cytosensor exhibited satisfying electrochemical behavior and cell-capture capacity for human colorectal cancer cells HCT-116, and also displayed a wide linear range, from 5.0 × 10² cells∙mL to 5.0 × 10⁶ cells∙mL, and a low detection limit of 100 cells∙mL (S/N = 3) for quantification. This work paves the way for graphene applications in electrochemical cytosensing and other bioassays.
用于早期肿瘤细胞检测的电化学传感器目前是一个重要的研究领域，因为这个特殊领域直接提高了癌症治疗的效率。功能化石墨烯是选择性识别和捕获目标癌细胞的一种有前景的替代材料。在我们的工作中，通过化学还原氧化石墨烯制备了一种有效的透明质酸功能化石墨烯（HG）细胞传感器。所制备的HG纳米结构分别通过傅里叶变换红外光谱、透射电子显微镜以及循环伏安图和电化学阻抗谱进行了表征。HG与乙二胺自组装，随后接上透明质酸钠，从而制备出了一种具有高稳定性和生物相容性的无标记电化学阻抗谱细胞传感器。最后，所提出的细胞传感器对人结肠癌细胞HCT - 116表现出令人满意的电化学行为和细胞捕获能力，并且还显示出宽线性范围，从5.0×10²个细胞∙mL到5.0×10⁶个细胞∙mL，以及用于定量的低检测限100个细胞∙mL（S/N = 3）。这项工作为石墨烯在电化学细胞传感和其他生物测定中的应用铺平了道路。
