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聚吡咯/石墨烯复合纳米纤维的合成与表征及其作为电化学 DNA 生物传感器在结核分枝杆菌检测中的应用。

Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis.

机构信息

Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia.

出版信息

Sensors (Basel). 2017 Dec 2;17(12):2789. doi: 10.3390/s17122789.

DOI:10.3390/s17122789
PMID:29207463
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5751647/
Abstract

This article describes chemically modified polyaniline and graphene (PANI/GP) composite nanofibers prepared by self-assembly process using oxidative polymerization of aniline monomer and graphene in the presence of a solution containing poly(methyl vinyl ether--maleic acid) (PMVEA). Characterization of the composite nanofibers was carried out by Fourier transform infrared (FTIR) and Raman spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). SEM images revealed the size of the PANI nanofibers ranged from 90 to 360 nm in diameter and was greatly influenced by the proportion of PMVEA and graphene. The composite nanofibers with an immobilized DNA probe were used for the detection of by using an electrochemical technique. A photochemical indicator, methylene blue (MB) was used to monitor the hybridization of target DNA by using differential pulse voltammetry (DPV) method. The detection range of DNA biosensor was obtained from of 10-10 M with the detection limit of 7.853 × 10 M under optimum conditions. The results show that the composite nanofibers have a great potential in a range of applications for DNA sensors.

摘要

本文描述了通过自组装过程制备的化学修饰的聚苯胺和石墨烯（PANI/GP）复合纳米纤维，该过程使用苯胺单体和石墨烯在含有聚（甲基乙烯基醚-马来酸）（PMVEA）的溶液中进行氧化聚合。通过傅里叶变换红外（FTIR）和拉曼光谱、透射电子显微镜（TEM）和扫描电子显微镜（SEM）对复合纳米纤维进行了表征。SEM 图像显示，PANI 纳米纤维的直径大小范围从 90nm 到 360nm，并且受 PMVEA 和石墨烯比例的影响很大。固定有 DNA 探针的复合纳米纤维用于通过电化学技术检测。使用亚甲基蓝（MB）作为光化学指示剂，通过差分脉冲伏安法（DPV）方法监测目标 DNA 的杂交。在最佳条件下，DNA 生物传感器的检测范围为 10-10 M，检测限为 7.853×10 M。结果表明，复合纳米纤维在 DNA 传感器的一系列应用中具有很大的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/16f57183d835/sensors-17-02789-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/25ba91e3314a/sensors-17-02789-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/b83c29951615/sensors-17-02789-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/0d380ae67dee/sensors-17-02789-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/e0c967d69608/sensors-17-02789-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/0e1c5621282e/sensors-17-02789-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/ffa5fd1e03e8/sensors-17-02789-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/d06310fff1fb/sensors-17-02789-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/5b6aca7c929e/sensors-17-02789-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/19bb8a8bb8b0/sensors-17-02789-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/232b144292d2/sensors-17-02789-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/f369d5b179d1/sensors-17-02789-g011a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/16f57183d835/sensors-17-02789-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/25ba91e3314a/sensors-17-02789-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/b83c29951615/sensors-17-02789-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/0d380ae67dee/sensors-17-02789-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/e0c967d69608/sensors-17-02789-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/0e1c5621282e/sensors-17-02789-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/ffa5fd1e03e8/sensors-17-02789-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/d06310fff1fb/sensors-17-02789-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/5b6aca7c929e/sensors-17-02789-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/19bb8a8bb8b0/sensors-17-02789-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/232b144292d2/sensors-17-02789-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/f369d5b179d1/sensors-17-02789-g011a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4072/5751647/16f57183d835/sensors-17-02789-g012.jpg

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