Sankar P, Shanmugam K T
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
J Bacteriol. 1988 Dec;170(12):5446-51. doi: 10.1128/jb.170.12.5446-5451.1988.
A new gene whose product is essential for production of all three hydrogenase isoenzymes in Escherichia coli has been identified. This gene, termed hydF, mapped at 59 min in the E. coli chromosome and resided next to the hydB gene. The map order of these genes was hydE, hydF, hydB, fhlA, and fdv. The hydF gene was transcribed from its own promoter and coded for a protein with an apparent molecular weight of 43,000 to 44,000. Expression of the hydF operon was enhanced by anaerobic growth conditions. Partial products of the hydF gene were capable of supporting various levels of hydrogenase activity in a hydF mutant in the presence of the fhlA gene product, also produced from multicopy plasmids. In the presence of a second mutation in an unidentified, unlinked gene, hydrogenase activity in a hydF mutant was restored by plasmids which carried incomplete hydF and hydB+ genes. These results suggest that the products of hydF and fhlA interact with each other and with yet one other gene product.
已鉴定出一个新基因，其产物对于大肠杆菌中所有三种氢化酶同工酶的产生至关重要。这个基因被称为hydF，定位于大肠杆菌染色体的59分钟处，位于hydB基因旁边。这些基因的图谱顺序是hydE、hydF、hydB、fhlA和fdv。hydF基因从其自身启动子转录，编码一种表观分子量为43,000至44,000的蛋白质。hydF操纵子的表达在厌氧生长条件下增强。在存在同样由多拷贝质粒产生的fhlA基因产物的情况下，hydF基因的部分产物能够在hydF突变体中支持不同水平的氢化酶活性。在一个未鉴定的、不连锁基因发生第二次突变的情况下，携带不完整hydF和hydB+基因的质粒可恢复hydF突变体中的氢化酶活性。这些结果表明，hydF和fhlA的产物相互作用，并与另一种基因产物相互作用。
