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大肠杆菌中氢代谢的生化与遗传分析：hydB基因

Biochemical and genetic analysis of hydrogen metabolism in Escherichia coli: the hydB gene.

作者信息

Sankar P, Shanmugam K T

机构信息

Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.

出版信息

J Bacteriol. 1988 Dec;170(12):5433-9. doi: 10.1128/jb.170.12.5433-5439.1988.

DOI:10.1128/jb.170.12.5433-5439.1988
PMID:2848007
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211634/
Abstract

Production of active hydrogenase by Escherichia coli requires several gene products. One of the essential genes, hydB, is encoded by a DNA fragment of approximately 1.0 kilobase. The hydB gene produced a protein with an apparent molecular weight of 32,000. The hydB gene was transcribed only under anaerobic conditions. Oxygen and nitrate repressed transcription of this gene. hydB gene transcription also required sigma 60, the product of the rpoN gene.

摘要

大肠杆菌产生活性氢化酶需要几种基因产物。其中一个必需基因hydB由一个约1.0千碱基的DNA片段编码。hydB基因产生一种表观分子量为32,000的蛋白质。hydB基因仅在厌氧条件下转录。氧气和硝酸盐会抑制该基因的转录。hydB基因转录还需要rpoN基因的产物sigma 60。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4177/211634/2703b14521e1/jbacter00190-0045-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4177/211634/8abbbdb3a4a7/jbacter00190-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4177/211634/2703b14521e1/jbacter00190-0045-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4177/211634/8abbbdb3a4a7/jbacter00190-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4177/211634/2703b14521e1/jbacter00190-0045-b.jpg

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