Qiao Zixu, Cao Min, Wang Dan, Liao Shuijiao, Wang Gejiao
1State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
2College of Basic Science, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Int J Syst Evol Microbiol. 2019 Feb;69(2):498-503. doi: 10.1099/ijsem.0.003186. Epub 2018 Dec 20.
A Gram-stain-negative, strictly aerobic bacterium, designated strain QZX222, was isolated from arsenic-contaminated farmland soil. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain QZX222 was clustered with Sphingosinicella vermicomposti YC7378 (97.0 %), Sphingosinicellaxenopeptidilytica 3-2W4 (96.1 %), Sphingosinicella microcystinivorans Y2 (96.0 %) and Sphingosinicella soli KSL-125 (95.9 %). Compared to strain QZX222, Spingomonas olgophenolica JCM 12082 and Sphingobium boeckii 469 had 16S rRNA gene similarities of 96.2 and 95.9 %, respectively, but they located in other phylogenetic clusters. DNA-DNA hybridization and genomic ANI values between strain QZX222 and Sphingosinicella vermicomposti DSM 21593 (KCTC 22446) were 34.8 and 75.0 %, respectively. The genome size of strain QZX222 was 3.0 Mb including 2982 predicted genes. The strain had a DNA G+C content of 65.9 mol%. Strain QZX222 had ubiquinone Q-10 as the major respiratory quinone and homospermidine as the major polyamine. The major fatty acids (>10 %) of strain QZX222 were C17 : 1ω6c, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C17 : 1ω8c. The polar lipids were sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and an unidentified glycolipid. Strain QZX222 could be distinguished from other Sphingosinicella strains based on the results of phylogenetic and genomic analyses, DNA-DNA hybridization, white colour colony, hydrolysis of urea, alkaline phosphatase activity, lack of phosphatidylmonomethylethanolamine, and presence of phosphatidylcholine. Therefore, strain QZX222 represents a novel species of Sphingosinicella, for which the name Sphingosinicellahumi sp. nov. is proposed. The type strain is QZX222 (=KCTC 62519=CCTCC AB 2018030).
从砷污染的农田土壤中分离出一株革兰氏染色阴性、严格需氧的细菌，命名为菌株QZX222。基于16S rRNA基因序列的系统发育分析表明，菌株QZX222与蚯蚓堆肥鞘氨醇单胞菌YC7378（97.0%）、解肽鞘氨醇单胞菌3-2W4（96.1%）、微囊藻鞘氨醇单胞菌Y2（96.0%）和土壤鞘氨醇单胞菌KSL-125（95.9%）聚类在一起。与菌株QZX222相比，寡酚鞘氨醇单胞菌JCM 12082和解磷鞘氨醇杆菌469的16S rRNA基因相似度分别为96.2%和95.9%，但它们位于其他系统发育簇中。菌株QZX222与蚯蚓堆肥鞘氨醇单胞菌DSM 21593（KCTC 22446）之间的DNA-DNA杂交和基因组ANI值分别为34.8%和75.0%。菌株QZX222的基因组大小为3.0 Mb，包含2982个预测基因。该菌株的DNA G+C含量为65.9 mol%。菌株QZX222以泛醌Q-10作为主要呼吸醌，以高亚精胺作为主要多胺。菌株QZX222的主要脂肪酸（>10%）为C17 : 1ω6c、总和特征8（C18 : 1ω7c和/或C18 : 1ω6c）和C17 : 1ω8c。极性脂质为鞘糖脂、磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、磷脂酰胆碱和一种未鉴定的糖脂。基于系统发育和基因组分析结果、DNA-DNA杂交、白色菌落、尿素水解情况、碱性磷酸酶活性、缺乏磷脂酰单甲基乙醇胺以及存在磷脂酰胆碱，菌株QZX222可与其他鞘氨醇单胞菌菌株区分开来。因此，菌株QZX222代表鞘氨醇单胞菌属的一个新物种，为此提出名称为土壤鞘氨醇单胞菌（Sphingosinicella humi sp. nov.）。模式菌株为QZX222（=KCTC 62519=CCTCC AB 2 The type strain is QZX222 (=KCTC 62519=CCTCC AB 2018030).
