Guo Yuze, Tang Kai, Yao Ting, Zhang Jieyuzheng, Liu Yang, Meng Jianyu, Feng Fuying
Laboratory of Environmental Microbiology and Technology in Arid and Cold Regions, College of Life Science, Inner Mongolia Agricultural University, Hohhot, 010020, PR China.
Curr Microbiol. 2025 Mar 1;82(4):163. doi: 10.1007/s00284-025-04124-1.
A Gram-stain-negative, aerobic, rod-shaped, and yellow-colored bacterium, designated strain GR2756, was isolated from a rhizosphere soil sample of oat (Avena sativa L.). The strain GR2756 was able to grow at 15-35 °C, pH 6.0-8.0, and 0-1.5 % (w/v) NaCl concentration. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain GR2756 was clustered with Sphingosinicella vermicomposti YC7378 (95.7 %), Sphingosinicella humi QZX222 (95.6 %), and Sphingomonas montis DRJ-4 (95.5 %). Strain GR2756 contained ubiquinone Q-10 and homospermidine as the major respiratory quinone system and polyamine, respectively. The major fatty acids detected from strain GR2756 were C, Summed Feature 3 (C ω7c and/or C ω6c), and Summed Feature 8 (C ω7c and/or C ω6c), C ω6c and C 2-OH. The major polar lipids are phosphatidylethanolamine (PE), phospholipid, glycolipid, and unidentified polar lipid. The genomic DNA G+C was 63.2%. Between the strains GR2756 and S. humi CCTCC AB 2018030, the ANI and dDDH values were lower than the proposed and generally accepted species boundaries. On the basis of phenotypic, genotypic, and phylogenetic analysis, strain GR2756 represents a novel species of the genus Sphingosinicella, for which the name Sphingosinicella rhizophila sp. nov. is proposed. The type strain of the proposed new taxon is strain GR2756 (= CCTCC AB 2023102 = CGMCC 1.61580 = NBRC 116723).
从燕麦（Avena sativa L.）根际土壤样品中分离出一株革兰氏阴性、需氧、杆状且呈黄色的细菌，命名为GR2756菌株。GR2756菌株能够在15 - 35℃、pH 6.0 - 8.0以及0 - 1.5%（w/v）NaCl浓度下生长。基于16S rRNA基因序列的系统发育分析表明，GR2756菌株与蚯蚓堆肥鞘氨醇单胞菌YC7378（95.7%）、潮湿鞘氨醇单胞菌QZX222（95.6%）和蒙氏鞘脂单胞菌DRJ - 4（95.5%）聚类在一起。GR2756菌株分别含有泛醌Q - 10和高亚精胺作为主要呼吸醌系统和多胺。从GR2756菌株中检测到的主要脂肪酸为C、特征性总和3（C ω7c和/或C ω6c）以及特征性总和8（C ω7c和/或C ω6c）、C ω6c和C 2 - OH。主要极性脂为磷脂酰乙醇胺（PE）、磷脂、糖脂和未鉴定的极性脂。基因组DNA的G + C含量为63.2%。在GR2756菌株和潮湿鞘氨醇单胞菌CCTCC AB 2018030之间，ANI和dDDH值低于提议的且普遍接受的种间界限。基于表型、基因型和系统发育分析，GR2756菌株代表鞘氨醇单胞菌属的一个新物种，为此提议将其命名为嗜根鞘氨醇单胞菌（Sphingosinicella rhizophila sp. nov.）。提议的新分类单元的模式菌株为GR2756菌株（= CCTCC AB 2023102 = CGMCC 1.61580 = NBRC 116723）。
