Wallace J S, Fleming K A, Tarin D
Nuffield Department of Pathology, University of Oxford, John Radcliffe Hospital, U.K.
J Pathol. 1988 Sep;156(1):73-6. doi: 10.1002/path.1711560114.
This study reports high incidence tumourigenic activity of the pSV2neo plasmid demonstrated by transfection of NIH 3T3 cells. The plasmid is frequently used as a dominant selectable marker for confirming successful gene transfection, and the findings indicate a need for caution in interpretation and design of assays for oncogenes which use co-transfection and subsequent selection with neomycin.
