Pospelova T V, Kapitskaia M Z, Dvorianchikov G A, Tomilin N V
Tsitologiia. 1986 Dec;28(12):1329-35.
Transfection of the plasmid containing the cloned gag-myc part of retrovirus MC 29 into mouse NIH 3T3 cells induces focuses of morphological transformation. Isolated morphological transformants have a decreased dependence on serum growth factors, a higher saturation density in monolayer, and an increased cloning efficiency on the glass and in agar. Induced traits are stably inherited, and may constitute the direct consequence of stable maintenance and expression of transfected oncogens.
将含有逆转录病毒MC 29克隆的gag-myc部分的质粒转染到小鼠NIH 3T3细胞中可诱导形态转化灶形成。分离得到的形态转化细胞对血清生长因子的依赖性降低，单层培养时的饱和密度更高，在玻璃和琼脂中的克隆效率增加。诱导的性状可稳定遗传，可能是转染癌基因稳定维持和表达的直接结果。
