College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Hepatobiliary Pancreat Dis Int. 2019 Feb;18(1):48-61. doi: 10.1016/j.hbpd.2018.12.001. Epub 2018 Dec 6.
Hydrogen-rich saline (HRS) has antioxidative, anti-inflammatory and anti-apoptotic properties. We investigated the effects of hydrogen on hepatic ischemia-reperfusion (I/R) and laparoscopic hepatectomy in swine.
Twenty-one healthy Bama miniature pigs were randomly divided into the sham group, ischemia-reperfusion injury (IRI) group, HRS-5 (5 mL/kg) group, and HRS-10 (10 mL/kg) group. HRS was injected through the portal vein 10 min before reperfusion and at postoperative day 1, 2 and 3. The roles of HRS on oxidative stress, inflammatory response and liver regeneration were studied.
Compared with the IRI group, HRS treatment attenuated oxidative stress by increasing catalase activity and reducing myeloperoxidase. White blood cells in the HRS-10 group were reduced compared with the IRI group (P < 0.01). In the HRS-10 group, interleukin-1 beta, interleukin-6 and tumor necrosis factor alpha, C-reactive protein and cortisol were downregulated, whereas interleukin-10 was upregulated. In addition, HRS attenuated endothelial cell injury and promoted the secretion of angiogenic cytokines, including vascular endothelial growth factor, angiopoietin-1 and angiopoietin-2. HRS elevated the levels of hepatocyte growth factor, Cyclin D1, proliferating cell nuclear antigen, Ki-67 and reduced the secretion of transforming growth factor-beta.
HRS treatment may exert a protective effect against I/R and hepatectomy-induced hepatic damage by reducing oxidative stress, suppressing the inflammatory response and promoting liver regeneration.
富氢生理盐水（HRS）具有抗氧化、抗炎和抗细胞凋亡作用。我们研究了氢气对猪肝缺血再灌注（I/R）和腹腔镜肝切除术的影响。
21 只健康的巴马小型猪随机分为假手术组、缺血再灌注损伤（IRI）组、HRS-5（5mL/kg）组和 HRS-10（10mL/kg）组。HRS 在再灌注前 10min 和术后第 1、2、3 天通过门静脉注射。研究了 HRS 对氧化应激、炎症反应和肝再生的作用。
与 IRI 组相比，HRS 治疗通过增加过氧化氢酶活性和减少髓过氧化物酶来减轻氧化应激。与 IRI 组相比，HRS-10 组的白细胞减少（P<0.01）。在 HRS-10 组中，白细胞介素-1β、白细胞介素-6 和肿瘤坏死因子-α、C 反应蛋白和皮质醇下调，而白细胞介素-10 上调。此外，HRS 减轻了内皮细胞损伤，促进了血管生成细胞因子的分泌，包括血管内皮生长因子、血管生成素-1 和血管生成素-2。HRS 升高了肝细胞生长因子、Cyclin D1、增殖细胞核抗原、Ki-67 的水平，并减少了转化生长因子-β的分泌。
HRS 治疗可能通过减轻氧化应激、抑制炎症反应和促进肝再生，对 I/R 和肝切除引起的肝损伤发挥保护作用。
