College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, PR China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, PR China.
Biomed Pharmacother. 2019 Dec;120:109453. doi: 10.1016/j.biopha.2019.109453. Epub 2019 Sep 24.
To compare and evaluate the hepatoprotective effect of liver parenchyma injection of ADSCs and portal vein injection of HRS in laparoscopic hepatic ischemia reperfusion combined with hepatectomy injury in miniature pigs.
Eighteen miniature pigs were randomly assigned to IRI group, HRS group and ADSCs group. HRS was injected through the portal vein 10 min before reperfusion, 1 d, 2 d, and 3 d after surgery. ADSCs were injected into liver parenchyma after hepatectomy. The serum and liver tissue samples were collected at different time points (preoperative, and postoperative at 1 d, 3 d and 7 d).
Compared with the IRI group, both ADSCs and HRS groups can promote liver function recovery, reduce oxidative stress, reduce inflammation, and promote liver regeneration. Compared with HRS, ALT and TBIL in ADSCs group were significantly decreased at 3 d, and AST was significantly reduced at 1 d. The activities of SOD and GSH-Px in ADSCs group were significantly higher than that in HRS group, but the MDA level in HRS group was markedly lower than that in ADSCs group at 1 d. IL-1β was significantly lower in the ADSCs group than in the HRS group at 1 day after operation. The expressions of HGF and PCNA were significantly higher than that in the HRS group at 3 day after surgery.
Our study has demonstrated that HRS and ADSCs have significant hepatoprotective effects in miniature pigs after HIRI and hepatectomy injury. However, liver parenchyma injection of ADSCs is more beneficial to the recovery of liver function than portal vein injection of HRS.
比较和评估 ADSC 肝实质内注射与 HRS 门静脉注射对腹腔镜肝缺血再灌注联合肝切除损伤小型猪的肝保护作用。
将 18 只小型猪随机分为 IRI 组、HRS 组和 ADSCs 组。HRS 在再灌注前 10 min 经门静脉注射，术后 1、2 和 3 d 各注射 1 次。ADSCs 在肝切除后注入肝实质。在不同时间点（术前、术后 1、3 和 7 d）采集血清和肝组织样本。
与 IRI 组相比，ADSCs 和 HRS 组均可促进肝功能恢复，减轻氧化应激，减轻炎症，促进肝再生。与 HRS 组相比，ADSCs 组术后 3 d 时 ALT 和 TBIL 明显降低，1 d 时 AST 明显降低。ADSCs 组 SOD 和 GSH-Px 活性明显高于 HRS 组，而 1 d 时 HRS 组 MDA 水平明显低于 ADSCs 组。术后 1 d，ADSCs 组 IL-1β明显低于 HRS 组。术后 3 d，ADSCs 组 HGF 和 PCNA 的表达明显高于 HRS 组。
本研究表明，HRS 和 ADSCs 对小型猪肝缺血再灌注和肝切除损伤均有明显的肝保护作用。然而，ADSCs 肝实质内注射比 HRS 门静脉注射更有利于肝功能的恢复。
