Betancor Mónica B, Olsen Rolf E, Marandel Lucie, Skulstad Ole F, Madaro Angelico, Tocher Douglas R, Panserat Stephane
Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling, United Kingdom.
Department of Biology, Norwegian University of Science and Technology, Trondheim, Norway.
Front Physiol. 2018 Dec 6;9:1751. doi: 10.3389/fphys.2018.01751. eCollection 2018.
A common-garden experiment was carried out to compare two genetically distinct strains of Atlantic salmon () fed diets with either high (CHO) or low (NoCHO) digestible carbohydrate (starch). Twenty salmon from either a commercial farmed strain (F) or a land-locked population (G) were placed in two tanks (10 fish of each population in each tank) and fed either CHO or NoCHO feeds. At the end of the experiment fish were fasted for 8 h, euthanized and blood and liver collected. Both diet and population had an effect on circulating glucose levels with G showing hypoglycaemia and dietary starch increasing this parameter. In contrast, G showed increased plasma triacylglycerol levels regardless of dietary treatment suggesting faster conversion of glucose to triacylglycerol. This different ability to metabolize dietary starch among strains was also reflected at a molecular (gene) level as most of the metabolic pathways evaluated were mainly affected by the factor population rather than by diet. The data are promising and suggest different regulatory capacities toward starch utilization between land-locked salmon and the farmed stock. Further analyses are necessary in order to fully characterize the capacity of land-locked salmon to utilize dietary carbohydrate.
开展了一项共同饲养实验，以比较两种基因不同的大西洋鲑鱼品系，给它们投喂高（CHO）或低（NoCHO）可消化碳水化合物（淀粉）的饲料。从商业养殖品系（F）或陆封种群（G）中选取20条鲑鱼，放入两个水箱（每个水箱中每个种群各10条鱼），分别投喂CHO或NoCHO饲料。实验结束时，让鱼禁食8小时，实施安乐死并采集血液和肝脏。饲料和种群均对循环葡萄糖水平有影响，G品系表现出低血糖，而饲料中的淀粉会增加这一参数。相反，无论饲料处理如何，G品系的血浆三酰甘油水平均升高，这表明葡萄糖向三酰甘油的转化更快。品系间这种代谢饲料淀粉的不同能力在分子（基因）水平上也有体现，因为所评估的大多数代谢途径主要受种群因素而非饲料的影响。这些数据很有前景，表明陆封鲑鱼和养殖种群对淀粉利用的调节能力不同。为了全面表征陆封鲑鱼利用饲料碳水化合物的能力，还需要进一步分析。
