GROW Research Laboratory, Narayana Nethralaya Foundation, Bengaluru, Karnataka, India.
Narayana Nethralaya Eye Hospital, Bengaluru, Karnataka, India.
Indian J Ophthalmol. 2019 Jan;67(1):38-41. doi: 10.4103/ijo.IJO_568_18.
To determine the levels of dopamine in tear fluid and demonstrate the use of tear fluid as a non-invasive source for dopamine measurements in humans.
The study cohort included 30 clinically healthy individuals without any pre-existing ocular or systemic conditions. Matched tear fluid (using Schirmer's strips and capillary tubes) and plasma were collected from the subjects. Dopamine levels were evaluated using direct competitive chemiluminescent enzyme-linked immunosorbent assay (ELISA), dopamine kit (Cloud Clone Corp, TX, USA).
Significantly higher dopamine levels were found in the tear fluid compared to plasma in the study subjects. The level of dopamine was 97.2 ± 11.80 pg/ml (mean ± SEM), 279 ± 14.8 pg/ml (mean ± SEM), and 470.4 ± 37.64 pg/ml (mean ± SEM) in the plasma and in the tears collected using Schirmer's strips and capillary tubes, respectively.
Dopamine was detectable in all the tear fluid samples tested and was also found to be at a higher concentration than in plasma samples. Tear fluid can be used as a non-invasive sample source to monitor dopamine levels.
测定泪液中的多巴胺水平，并证明泪液可作为一种非侵入性的人类多巴胺测量来源。
研究对象包括 30 名无任何先前存在的眼部或系统性疾病的临床健康个体。使用 Schirmer 条和毛细管从受试者中收集匹配的泪液（使用 Schirmer 条和毛细管）和血浆。使用直接竞争化学发光酶联免疫吸附测定（ELISA）、多巴胺试剂盒（Cloud Clone Corp，TX，USA）评估多巴胺水平。
与血浆相比，研究对象的泪液中的多巴胺水平明显更高。多巴胺的水平分别为血浆中 97.2 ± 11.80 pg/ml（平均值 ± SEM）、Schirmer 条收集的泪液中 279 ± 14.8 pg/ml（平均值 ± SEM）和毛细管收集的泪液中 470.4 ± 37.64 pg/ml（平均值 ± SEM）。
在所有测试的泪液样本中均检测到多巴胺，并且发现其浓度也高于血浆样本。泪液可作为非侵入性样本来源，用于监测多巴胺水平。
