Maizels R M, Morgan T M, Gregory W F, Selkirk M E, Partono F
Department of Pure and Applied Biology, Imperial College of Science and Technology, London, UK.
Trop Med Parasitol. 1988 Sep;39(3):214-20.
Levels of circulating filarial antigen, and humoral antibody to other defined antigenic targets, were measured over the course of experimental infection of three Presbytis cristatus monkeys with Wuchereria bancrofti. Circulating antigen levels, measured with an anti-phosphorylcholine monoclonal antibody, varied widely although all animals were positive for some period of the infection. Circulating antigen levels tended to be inversely related to the titre of anti-phosphorylcholine antibody, and this trend was maintained even following acid dissociation and inactivation of immune complexed host antibody. Other antibody specificities were measured by Western blotting against somatic proteins and immunoprecipitation of surface antigens. Amongst somatic antigens, targets between 14,000 and 200,000 daltons were recognised by monkey antibodies, but no correlation with infection status could be discerned. Likewise when measuring the response to the 29,000 dalton major adult surface glycoprotein, one animal produced a rapid response but the others did not recognise it until late in infection. In general, the experimental findings are characterised by wide variability between individuals, as may perhaps be expected in a primate host for a human spectral disease.
在用班氏吴策线虫感染三只冠叶猴的实验过程中，检测了循环丝虫抗原水平以及针对其他特定抗原靶点的体液抗体。使用抗磷酸胆碱单克隆抗体检测的循环抗原水平差异很大，尽管所有动物在感染的某些阶段均呈阳性。循环抗原水平往往与抗磷酸胆碱抗体滴度呈负相关，即使在免疫复合物宿主抗体经酸解离和灭活后，这种趋势仍保持不变。通过针对体细胞蛋白的蛋白质印迹法和表面抗原的免疫沉淀法检测其他抗体特异性。在体细胞抗原中，猴子抗体识别出分子量在14,000至200,000道尔顿之间的靶点，但未发现与感染状态有相关性。同样，在检测对29,000道尔顿主要成虫表面糖蛋白的反应时，一只动物产生了快速反应，而其他动物直到感染后期才识别出该蛋白。总体而言，实验结果的特点是个体之间差异很大，这在人类谱系疾病的灵长类宿主中可能是可以预料的。
