Department of Basic Sciences, Division of Biomedical Engineering Sciences, Loma Linda University School of Medicine, Loma Linda, CA 92354, USA.
Department of Biochemistry, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA.
Int J Mol Sci. 2018 Dec 20;20(1):7. doi: 10.3390/ijms20010007.
There is evidence that spaceflight poses acute and late risks to the central nervous system. To explore possible mechanisms, the proteomic changes following spaceflight in mouse brain were characterized. Space Shuttle Atlantis (STS-135) was launched from the Kennedy Space Center (KSC) on a 13-day mission. Within 3⁻5 h after landing, brain tissue was collected to evaluate protein expression profiles using quantitative proteomic analysis. Our results showed that there were 26 proteins that were significantly altered after spaceflight in the gray and/or white matter. While there was no overlap between the white and gray matter in terms of individual proteins, there was overlap in terms of function, synaptic plasticity, vesical activity, protein/organelle transport, and metabolism. Our data demonstrate that exposure to the spaceflight environment induces significant changes in protein expression related to neuronal structure and metabolic function. This might lead to a significant impact on brain structural and functional integrity that could affect the outcome of space missions.
有证据表明，航天飞行会对中枢神经系统造成急性和晚期风险。为了探索可能的机制，本研究对小鼠脑在航天飞行后的蛋白质组变化进行了特征分析。亚特兰蒂斯号航天飞机（STS-135）从肯尼迪航天中心（KSC）发射，进行了为期 13 天的任务。着陆后 3⁻5 小时内，采集脑组织，使用定量蛋白质组分析评估蛋白质表达谱。我们的结果表明，在灰色和/或白色物质中，有 26 种蛋白质在航天飞行后发生了显著改变。虽然在单个蛋白质方面，白色和灰色物质之间没有重叠，但在功能、突触可塑性、囊泡活性、蛋白质/细胞器运输和代谢方面存在重叠。我们的数据表明，暴露于航天飞行环境会引起与神经元结构和代谢功能相关的蛋白质表达的显著变化。这可能会对大脑的结构和功能完整性产生重大影响，从而影响航天任务的结果。
