Ohno T, Seki M, Shikita M
Blood. 1978 May;51(5):911-8.
Colony-stimulating factors (CSF) active on both human and murine bone marrow colony-forming units in culture (CFU-C) were found in the conditioned medium of Yoshida sarcoma cells (line YSSF-212T), although the cells originated from rats. The CSF were inactivated by digestion with trypsin, alpha-chymotrypsin, and pronase. By chromatography on DEAE-cellulose, the CSF were separated into five subgroups with different capacities to stimulate mouse granulocyte and macrophage progenitors. CSF in these five peaks were eluted from an Ultrogel AcA 44 gel filtration column with apparent molecular weights of 22,000-25,000 daltons. CSF of nonhuman origin stimulating human CFU-C would be useful in hematologic studies of bone marrow cells.
尽管吉田肉瘤细胞（YSSF - 212T系）源自大鼠，但在其条件培养基中发现了对培养中的人及小鼠骨髓集落形成单位（CFU - C）有活性的集落刺激因子（CSF）。这些CSF可被胰蛋白酶、α - 糜蛋白酶和链霉蛋白酶消化而失活。通过DEAE - 纤维素柱层析，CSF被分离成五个亚组，它们刺激小鼠粒细胞和巨噬细胞祖细胞的能力各不相同。这五个峰中的CSF从Ultrogel AcA 44凝胶过滤柱上洗脱下来，其表观分子量为22,000 - 25,000道尔顿。非人类来源的刺激人CFU - C的CSF在骨髓细胞的血液学研究中会很有用。
