Center of Innovative and Applied Bioprocessing (CIAB), Sector-81 (Knowledge City), Mohali 140306, Punjab, India.
Center of Innovative and Applied Bioprocessing (CIAB), Sector-81 (Knowledge City), Mohali 140306, Punjab, India; Academy of Scientific and Innovative Research (AcSIR), Sector 19, Kamla Nehru Nagar, Ghaziabad, Uttar Pradesh 201 002, India.
Bioresour Technol. 2019 Mar;275:430-433. doi: 10.1016/j.biortech.2018.12.042. Epub 2018 Dec 14.
In the present investigation, several residues from agro-forestry industries such as rice straw acid hydrolysate, corn cob acid hydrolysate, tomato juice, cane molasses and orange pulp were evaluated as the economical source for the production of bacterial cellulose. The bacterial cellulose attained the significant yield of 7.8 g/L using tomato juice, followed by 3.6 g/L using cane molasses and 2.8 g/L using orange pulp after 7 days of incubation. Furthermore, the optimum pH and temperature of fermentation for maximum production of bacterial cellulose was 4.5 and 30 ± 1 °C. The identified bacterium Acetobacter pasteurianus RSV-4 has been deposited at repository under the accession number MTCC 25117. The produced bacterial cellulose was characterized through FTIR, SEM, TGA and DSC and found to be of very good quality. The bacterial cellulose produced by identified strain on these various agro-waste residues could be a cost effective technology for commercial its production.
在本研究中，评估了农业林业产业的几种残留物，如稻草酸水解物、玉米芯酸水解物、番茄汁、甘蔗糖蜜和橙浆，作为生产细菌纤维素的经济来源。用番茄汁培养 7 天后，细菌纤维素的产量达到了显著的 7.8g/L，其次是用甘蔗糖蜜培养的 3.6g/L，用橙浆培养的 2.8g/L。此外，细菌纤维素产量最大的发酵最佳 pH 值和温度为 4.5 和 30±1°C。鉴定出的醋杆菌属巴氏亚种 RSV-4 已在 MTCC 25117 登记号下的储存库中保存。通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、热重分析（TGA）和差示扫描量热法（DSC）对所生产的细菌纤维素进行了表征，发现其质量非常好。在这些各种农业废物残留物上由鉴定出的菌株生产的细菌纤维素可以是一种具有成本效益的商业生产技术。
