Bertocchi C, Delneri D, Signore S, Weng Z, Bruschi C V
Poly-tech S.C.ar.l., AREA Science Park, Trieste, Italy.
Biochim Biophys Acta. 1997 Aug 29;1336(2):211-7. doi: 10.1016/s0304-4165(97)00030-5.
A wild-type Acetobacter pasteurianus was subjected to chemical mutagenesis for the induction and isolation of a cellulose overproducing strain. A mutagenized strain capable of synthesizing double amounts of cellulose compared to the wild type was obtained. Cellulose, both from the wild-type and the mutagenized strain, was extracted and purified for chemical characterization and investigation of its physico-chemical properties. The comparison of the two microbial polysaccharides shows that the putative mutation of A. pasteurianus strain had no effect on some cellulose features such as chemical structure, polymorphic form, crystallinity.
对一株野生型巴氏醋杆菌进行化学诱变，以诱导和分离纤维素高产菌株。获得了一种诱变菌株，与野生型相比，其能够合成双倍量的纤维素。从野生型和诱变菌株中提取并纯化纤维素，用于化学表征及其物理化学性质的研究。两种微生物多糖的比较表明，巴氏醋杆菌菌株的推定突变对纤维素的某些特性如化学结构、多晶型、结晶度没有影响。
