Center of Innovative and Applied Bioprocessing (CIAB), Sector-81 (Knowledge City), Mohali 140306, PB, India.
Center of Innovative and Applied Bioprocessing (CIAB), Sector-81 (Knowledge City), Mohali 140306, PB, India.
Int J Biol Macromol. 2020 Aug 1;156:598-607. doi: 10.1016/j.ijbiomac.2020.04.078. Epub 2020 Apr 17.
There is ongoing quest to look for alternate sustainable and renewable biopolymers which can address the existing environmental issues. Bacterial cellulose could be one such option. Several organisms have been reported to produce bacterial cellulose. Among this, acetic acid bacteria (AAB) are reported to be one of the major producers of bacterial cellulose. Recently, we have identified an Acetobacter pasteurianus RSV-4 and reported to produce high tensile strength bacterial cellulose. In order to globally understand its genetic structure, a draft genome sequence of Acetobacter pasteurianus RSV-4 was performed in the present study. The assembled genome had 101 contigs contributing to a total length of 3.8 Mbp. Predicted coding DNA sequences were 3311, of which approximately 70% were assigned the functions. Genome level phylogenetic analysis revealed that RSV-4 belongs to A. pasteurianus. Glycolysis was found to be incomplete in the genome analysis of RSV-4, while the genes/enzymes involved in pentose-phosphate pathway were present. The final draft genome sequence lacked bacterial cellulose synthase (bcs) operon. However, the presence of operon was evident in raw genomic sequences by Sanger sequencing. Therefore, presence of bcs operon in Acetobacter pasteurianus RSV-4 has documented its potential for bacterial cellulose production.
目前，人们一直在探索替代性的可持续和可再生生物聚合物，以解决当前的环境问题。细菌纤维素就是这样一种选择。已经有报道称几种生物能够产生细菌纤维素。在这些生物中，醋酸菌（AAB）被认为是细菌纤维素的主要生产者之一。最近，我们已经鉴定出一株巴氏醋酸杆菌 RSV-4，并且报道称它能够产生高强度的细菌纤维素。为了在全球范围内了解其遗传结构，本研究对巴氏醋酸杆菌 RSV-4 进行了草图基因组序列分析。组装后的基因组由 101 个重叠群组成，总长度为 3.8 Mbp。预测的编码 DNA 序列有 3311 个，其中约 70%被赋予了功能。基因组水平的系统发育分析表明，RSV-4 属于巴氏醋酸杆菌。在 RSV-4 的基因组分析中发现糖酵解不完全，而戊糖磷酸途径相关的基因/酶存在。最终的草图基因组序列缺乏细菌纤维素合成酶（bcs）操纵子。然而，通过 Sanger 测序，在原始基因组序列中可以明显看出该操纵子的存在。因此，巴氏醋酸杆菌 RSV-4 中存在 bcs 操纵子，证明了其具有生产细菌纤维素的潜力。
