Sun D, Courtney H S, Beachey E H
Veterans Administration Medical Center, Memphis, Tennessee.
Antimicrob Agents Chemother. 1988 Sep;32(9):1370-4. doi: 10.1128/AAC.32.9.1370.
Berberine sulfate is an alkaloid extracted from the roots and bark of various plants and possesses antibacterial, antifungal, and antiprotozoal activities. Most studies have focused on the bacteriostatic or bactericidal activities of this compound. In this study, we report that berberine sulfate is bacteriostatic for streptococci and that sub-MICs of berberine blocked the adherence of streptococci to host cells, immobilized fibronectin, and hexadecane. Concentrations of berberine below its MIC caused an eightfold increase in release of lipoteichoic acid from the streptococci. Higher concentrations of berberine directly interfered with the adherence of streptococci to host cells either by preventing the complexing of lipoteichoic acid with fibronectin or by dissolution of such complexes once they were formed. Thus, berberine sulfate interferes with the adherence of group A streptococci by two distinct mechanisms: one by releasing the adhesin lipoteichoic acid from the streptococcal cell surface and another by directly preventing or dissolving lipoteichoic acid-fibronectin complexes.
硫酸小檗碱是从多种植物的根和树皮中提取的一种生物碱，具有抗菌、抗真菌和抗原虫活性。大多数研究都集中在该化合物的抑菌或杀菌活性上。在本研究中，我们报告硫酸小檗碱对链球菌具有抑菌作用，且小檗碱的亚抑菌浓度可阻断链球菌对宿主细胞的黏附、固定纤连蛋白和十六烷。低于其最低抑菌浓度的小檗碱浓度会使链球菌的脂磷壁酸释放量增加八倍。较高浓度的小檗碱直接干扰链球菌对宿主细胞的黏附，要么通过阻止脂磷壁酸与纤连蛋白的复合，要么在复合物形成后将其溶解。因此，硫酸小檗碱通过两种不同机制干扰A组链球菌的黏附：一种是从链球菌细胞表面释放黏附素脂磷壁酸，另一种是直接阻止或溶解脂磷壁酸 - 纤连蛋白复合物。
